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• W3072280779 endingPage "528" @default.
• W3072280779 startingPage "528" @default.
• W3072280779 abstract "Borrelia burgdorferi, the causative agent of Lyme disease, has been recently shown to form biofilm structures in vitro and in vivo. Biofilms are tightly clustered microbes characterized as resistant aggregations that allow bacteria to withstand harsh environmental conditions, including the administration of antibiotics. Novel antibiotic combinations have recently been identified for B. burgdorferi in vitro, however, due to prohibiting costs, those agents have not been tested in an environment that can mimic the host tissue. Therefore, researchers cannot evaluate their true effectiveness against B. burgdorferi, especially its biofilm form. A skin ex vivo model system could be ideal for these types of experiments due to its cost effectiveness, reproducibility, and ability to investigate host–microbial interactions. Therefore, the main goal of this study was the establishment of a novel ex vivo murine skin biopsy model for B. burgdorferi biofilm research. Murine skin biopsies were inoculated with B. burgdorferi at various concentrations and cultured in different culture media. Two weeks post-infection, murine skin biopsies were analyzed utilizing immunohistochemical (IHC), reverse transcription PCR (RT-PCR), and various microscopy methods to determine B. burgdorferi presence and forms adopted as well as whether it remained live in the skin tissue explants. Our results showed that murine skin biopsies inoculated with 1 × 107 cells of B. burgdorferi and cultured in BSK-H + 6% rabbit serum media for two weeks yielded not just significant amounts of live B. burgdorferi spirochetes but biofilm forms as well. IHC combined with confocal and atomic force microscopy techniques identified specific biofilm markers and spatial distribution of B. burgdorferi aggregates in the infected skin tissues, confirming that they are indeed biofilms. In the future, this ex vivo skin model can be used to study development and antibiotic susceptibility of B. burgdorferi biofilms in efforts to treat Lyme disease effectively." @default.
• W3072280779 created "2020-08-24" @default.
• W3072280779 creator A5012577517 @default.
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• W3072280779 creator A5058787969 @default.
• W3072280779 date "2020-08-19" @default.
• W3072280779 modified "2023-09-27" @default.
• W3072280779 title "Ex Vivo Murine Skin Model for B. burgdorferi Biofilm" @default.
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• W3072280779 cites W173949380 @default.
• W3072280779 cites W176162767 @default.
• W3072280779 cites W1971255678 @default.
• W3072280779 cites W1972590790 @default.
• W3072280779 cites W1980984820 @default.
• W3072280779 cites W1982136452 @default.
• W3072280779 cites W1986519128 @default.
• W3072280779 cites W1986683117 @default.
• W3072280779 cites W1989942811 @default.
• W3072280779 cites W1993369443 @default.
• W3072280779 cites W1995024953 @default.
• W3072280779 cites W2000954422 @default.
• W3072280779 cites W2005813123 @default.
• W3072280779 cites W2015096711 @default.
• W3072280779 cites W2025609755 @default.
• W3072280779 cites W2028161864 @default.
• W3072280779 cites W2030317740 @default.
• W3072280779 cites W2031937694 @default.
• W3072280779 cites W2032064628 @default.
• W3072280779 cites W2036058330 @default.
• W3072280779 cites W2036596008 @default.
• W3072280779 cites W2061126046 @default.
• W3072280779 cites W2068522004 @default.
• W3072280779 cites W2073372566 @default.
• W3072280779 cites W2087254697 @default.
• W3072280779 cites W2088540532 @default.
• W3072280779 cites W2095291804 @default.
• W3072280779 cites W2100553781 @default.
• W3072280779 cites W2103455329 @default.
• W3072280779 cites W2104202918 @default.
• W3072280779 cites W2104702791 @default.
• W3072280779 cites W2109434556 @default.
• W3072280779 cites W2110700973 @default.
• W3072280779 cites W2130803530 @default.
• W3072280779 cites W2136310274 @default.
• W3072280779 cites W2137123557 @default.
• W3072280779 cites W2139885941 @default.
• W3072280779 cites W2142463523 @default.
• W3072280779 cites W2143628550 @default.
• W3072280779 cites W2144440398 @default.
• W3072280779 cites W2149307040 @default.
• W3072280779 cites W2149908911 @default.
• W3072280779 cites W2159764553 @default.
• W3072280779 cites W2160674838 @default.
• W3072280779 cites W2163533527 @default.
• W3072280779 cites W2163586341 @default.
• W3072280779 cites W2180283734 @default.
• W3072280779 cites W2195522236 @default.
• W3072280779 cites W2207554282 @default.
• W3072280779 cites W2254366055 @default.
• W3072280779 cites W2273446410 @default.
• W3072280779 cites W2462524555 @default.
• W3072280779 cites W2529855748 @default.
• W3072280779 cites W2550795809 @default.
• W3072280779 cites W2554834191 @default.
• W3072280779 cites W2574343446 @default.
• W3072280779 cites W2590136055 @default.
• W3072280779 cites W2594019989 @default.
• W3072280779 cites W2600560956 @default.
• W3072280779 cites W2603755876 @default.
• W3072280779 cites W2605810129 @default.
• W3072280779 cites W2769793976 @default.
• W3072280779 cites W2770250225 @default.
• W3072280779 cites W2774068836 @default.
• W3072280779 cites W2789728425 @default.
• W3072280779 cites W2913885737 @default.
• W3072280779 cites W2936369607 @default.
• W3072280779 cites W2943627940 @default.
• W3072280779 cites W2946365367 @default.
• W3072280779 cites W2947856274 @default.
• W3072280779 cites W2948981686 @default.
• W3072280779 cites W2951290297 @default.
• W3072280779 cites W2952496405 @default.
• W3072280779 cites W2980149851 @default.
• W3072280779 cites W2993487502 @default.
• W3072280779 cites W3010117448 @default.
• W3072280779 cites W3013434973 @default.
• W3072280779 doi "https://doi.org/10.3390/antibiotics9090528" @default.
• W3072280779 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7558507" @default.
• W3072280779 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32824942" @default.
• W3072280779 hasPublicationYear "2020" @default.
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