Matches in SemOpenAlex for { <https://semopenalex.org/work/W3076551716> ?p ?o ?g. }
- W3076551716 endingPage "30699" @default.
- W3076551716 startingPage "30692" @default.
- W3076551716 abstract "Extracellular neutral proteases (NPs) in Aspergillus oryzae (A. oryzae) play a role in hydrolyzing soybean proteins into smaller peptides at pH about 7.5. The optimum pH of moromi fermentation (The second stage of soy sauce fermentation.) is 4.5-5.5. NPI is acid sensitive. To decrease the pH optimum of NPI, we got a mutant NPI-Y122FK246ID382V from the error-prone PCR library that showed optimal activity at pH 5.5. The specific activity at 40 °C of the NPI-Y122FK246ID382V mutant was 1383.50 U mg-1, which was 2.75-fold that of wild-type (503.09 U mg-1). The Michaelis constants of the mutant decreased from 22.13 mM (wild-type) to 19.98 mM (NPI-Y122FK246ID382V). The residues at positions 122 and 246 are important in influencing hydrolytic activity at pH 5.5 through site-directed mutagenesis. And the pH optimum of double amino acid mutants (Y122FK246I) shifted dramatically to an acidic pH compared to those of single amino acid substitution. Molecular models and structural comparisons of native and mutant provided further insight on the basis to improve catalytic efficiency at acidic pH. These results indicated that we modified the neutral protease I of Aspergillus oryzae, which can effectively improve the application of the neutral protease in industrial production, and finally lay the foundation for improving the utilization rate of raw protein." @default.
- W3076551716 created "2020-08-24" @default.
- W3076551716 creator A5020821336 @default.
- W3076551716 creator A5028495299 @default.
- W3076551716 creator A5035183903 @default.
- W3076551716 creator A5047266782 @default.
- W3076551716 creator A5075928672 @default.
- W3076551716 creator A5091589383 @default.
- W3076551716 date "2020-01-01" @default.
- W3076551716 modified "2023-10-17" @default.
- W3076551716 title "Engineering a recombination neutral protease I from <i>Aspergillus oryzae</i> to improve enzyme activity at acidic pH" @default.
- W3076551716 cites W1965641600 @default.
- W3076551716 cites W1975760680 @default.
- W3076551716 cites W1984465585 @default.
- W3076551716 cites W1989580463 @default.
- W3076551716 cites W1990591017 @default.
- W3076551716 cites W1998029045 @default.
- W3076551716 cites W2001049522 @default.
- W3076551716 cites W2013826392 @default.
- W3076551716 cites W2020971796 @default.
- W3076551716 cites W2027012851 @default.
- W3076551716 cites W2029327132 @default.
- W3076551716 cites W2030135851 @default.
- W3076551716 cites W2041114202 @default.
- W3076551716 cites W2042484942 @default.
- W3076551716 cites W2061038863 @default.
- W3076551716 cites W2061132174 @default.
- W3076551716 cites W2068459009 @default.
- W3076551716 cites W2069909193 @default.
- W3076551716 cites W2071430019 @default.
- W3076551716 cites W2075922243 @default.
- W3076551716 cites W2087235930 @default.
- W3076551716 cites W2090076234 @default.
- W3076551716 cites W2102033493 @default.
- W3076551716 cites W2102843616 @default.
- W3076551716 cites W2104096584 @default.
- W3076551716 cites W2106861615 @default.
- W3076551716 cites W2112169196 @default.
- W3076551716 cites W2135636849 @default.
- W3076551716 cites W2144703779 @default.
- W3076551716 cites W2155201844 @default.
- W3076551716 cites W2158248653 @default.
- W3076551716 cites W2316253106 @default.
- W3076551716 cites W2504490155 @default.
- W3076551716 cites W2725751175 @default.
- W3076551716 cites W2766460411 @default.
- W3076551716 cites W2769483918 @default.
- W3076551716 cites W2783289757 @default.
- W3076551716 cites W2905606194 @default.
- W3076551716 cites W2914639527 @default.
- W3076551716 cites W2924335595 @default.
- W3076551716 cites W4241127335 @default.
- W3076551716 cites W608896015 @default.
- W3076551716 doi "https://doi.org/10.1039/d0ra05462c" @default.
- W3076551716 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35516032" @default.
- W3076551716 hasPublicationYear "2020" @default.
- W3076551716 type Work @default.
- W3076551716 sameAs 3076551716 @default.
- W3076551716 citedByCount "5" @default.
- W3076551716 countsByYear W30765517162022 @default.
- W3076551716 countsByYear W30765517162023 @default.
- W3076551716 crossrefType "journal-article" @default.
- W3076551716 hasAuthorship W3076551716A5020821336 @default.
- W3076551716 hasAuthorship W3076551716A5028495299 @default.
- W3076551716 hasAuthorship W3076551716A5035183903 @default.
- W3076551716 hasAuthorship W3076551716A5047266782 @default.
- W3076551716 hasAuthorship W3076551716A5075928672 @default.
- W3076551716 hasAuthorship W3076551716A5091589383 @default.
- W3076551716 hasBestOaLocation W30765517161 @default.
- W3076551716 hasConcept C100544194 @default.
- W3076551716 hasConcept C104317684 @default.
- W3076551716 hasConcept C143065580 @default.
- W3076551716 hasConcept C147816474 @default.
- W3076551716 hasConcept C16318435 @default.
- W3076551716 hasConcept C181199279 @default.
- W3076551716 hasConcept C182220744 @default.
- W3076551716 hasConcept C185592680 @default.
- W3076551716 hasConcept C207583985 @default.
- W3076551716 hasConcept C2776714187 @default.
- W3076551716 hasConcept C2781204770 @default.
- W3076551716 hasConcept C3018936355 @default.
- W3076551716 hasConcept C515207424 @default.
- W3076551716 hasConcept C55493867 @default.
- W3076551716 hasConcept C9418097 @default.
- W3076551716 hasConcept C94412978 @default.
- W3076551716 hasConceptScore W3076551716C100544194 @default.
- W3076551716 hasConceptScore W3076551716C104317684 @default.
- W3076551716 hasConceptScore W3076551716C143065580 @default.
- W3076551716 hasConceptScore W3076551716C147816474 @default.
- W3076551716 hasConceptScore W3076551716C16318435 @default.
- W3076551716 hasConceptScore W3076551716C181199279 @default.
- W3076551716 hasConceptScore W3076551716C182220744 @default.
- W3076551716 hasConceptScore W3076551716C185592680 @default.
- W3076551716 hasConceptScore W3076551716C207583985 @default.
- W3076551716 hasConceptScore W3076551716C2776714187 @default.
- W3076551716 hasConceptScore W3076551716C2781204770 @default.
- W3076551716 hasConceptScore W3076551716C3018936355 @default.
- W3076551716 hasConceptScore W3076551716C515207424 @default.