Matches in SemOpenAlex for { <https://semopenalex.org/work/W3080165764> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W3080165764 abstract "Therapeutic applications of plasmid DNA (pDNA) have significantly advanced during the last years. Currently, several pDNA-based drugs are already in the market, whereas several others have entered phases 2 and 3 of clinical trials. The present and future demand for pDNA requires the development of efficient bioprocesses to produce it. Commonly, pDNA is produced by cultures of Escherichia coli. It has been previously demonstrated that specific strains of E. coli with a modified substrate transport system can be able to attain high cell densities in batch mode, due to the very low overflow metabolism displayed. However, the large amounts of oxygen demanded can lead to microaerobic conditions after some hours of cultivation, even at small scale. Typically, the inherent problems for these cultures are the high oxygen demand and the accumulation of acetate, a metabolic byproduct that is synthesized aerobically when the glucose rate exceeds the limits.In recent years, several researches have been focused on the study of induction of plasmid DNA as well as strategies for fermentation using semi-defined mediums. These studies conceived relevant results that allow us to design a production platform for enhanced plasmid DNA. So, the main goal of this chapter is to show how the development of an experimental design directed to aromatic amino acids pathway can improve the yield of a therapeutic plasmid DNA by culture of a new strain of Escherichia coli VH33." @default.
- W3080165764 created "2020-09-01" @default.
- W3080165764 creator A5049252324 @default.
- W3080165764 date "2020-08-22" @default.
- W3080165764 modified "2023-09-23" @default.
- W3080165764 title "Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design" @default.
- W3080165764 cites W1973125157 @default.
- W3080165764 cites W1977310162 @default.
- W3080165764 cites W1988275620 @default.
- W3080165764 cites W2003142776 @default.
- W3080165764 cites W2014805757 @default.
- W3080165764 cites W2017224563 @default.
- W3080165764 cites W2029822304 @default.
- W3080165764 cites W2046388139 @default.
- W3080165764 cites W2047515099 @default.
- W3080165764 cites W2058081044 @default.
- W3080165764 cites W2065818546 @default.
- W3080165764 cites W2066314519 @default.
- W3080165764 cites W2066947166 @default.
- W3080165764 cites W2084518511 @default.
- W3080165764 cites W2085796061 @default.
- W3080165764 cites W2088376206 @default.
- W3080165764 cites W2092735097 @default.
- W3080165764 cites W2095598803 @default.
- W3080165764 cites W2122031292 @default.
- W3080165764 cites W2126185220 @default.
- W3080165764 cites W2136786890 @default.
- W3080165764 cites W2137957492 @default.
- W3080165764 cites W2145786570 @default.
- W3080165764 cites W2145907180 @default.
- W3080165764 cites W2160511811 @default.
- W3080165764 cites W2171493278 @default.
- W3080165764 cites W2983048398 @default.
- W3080165764 doi "https://doi.org/10.1007/978-1-0716-0872-2_7" @default.
- W3080165764 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32827135" @default.
- W3080165764 hasPublicationYear "2020" @default.
- W3080165764 type Work @default.
- W3080165764 sameAs 3080165764 @default.
- W3080165764 citedByCount "0" @default.
- W3080165764 crossrefType "book-chapter" @default.
- W3080165764 hasAuthorship W3080165764A5049252324 @default.
- W3080165764 hasConcept C100544194 @default.
- W3080165764 hasConcept C104317684 @default.
- W3080165764 hasConcept C185592680 @default.
- W3080165764 hasConcept C22744801 @default.
- W3080165764 hasConcept C523546767 @default.
- W3080165764 hasConcept C54355233 @default.
- W3080165764 hasConcept C547475151 @default.
- W3080165764 hasConcept C552990157 @default.
- W3080165764 hasConcept C55493867 @default.
- W3080165764 hasConcept C86803240 @default.
- W3080165764 hasConcept C89423630 @default.
- W3080165764 hasConceptScore W3080165764C100544194 @default.
- W3080165764 hasConceptScore W3080165764C104317684 @default.
- W3080165764 hasConceptScore W3080165764C185592680 @default.
- W3080165764 hasConceptScore W3080165764C22744801 @default.
- W3080165764 hasConceptScore W3080165764C523546767 @default.
- W3080165764 hasConceptScore W3080165764C54355233 @default.
- W3080165764 hasConceptScore W3080165764C547475151 @default.
- W3080165764 hasConceptScore W3080165764C552990157 @default.
- W3080165764 hasConceptScore W3080165764C55493867 @default.
- W3080165764 hasConceptScore W3080165764C86803240 @default.
- W3080165764 hasConceptScore W3080165764C89423630 @default.
- W3080165764 hasLocation W30801657641 @default.
- W3080165764 hasOpenAccess W3080165764 @default.
- W3080165764 hasPrimaryLocation W30801657641 @default.
- W3080165764 hasRelatedWork W10545432 @default.
- W3080165764 hasRelatedWork W13784270 @default.
- W3080165764 hasRelatedWork W16009215 @default.
- W3080165764 hasRelatedWork W16773633 @default.
- W3080165764 hasRelatedWork W17335418 @default.
- W3080165764 hasRelatedWork W19354293 @default.
- W3080165764 hasRelatedWork W20487002 @default.
- W3080165764 hasRelatedWork W38543 @default.
- W3080165764 hasRelatedWork W5106937 @default.
- W3080165764 hasRelatedWork W11830727 @default.
- W3080165764 isParatext "false" @default.
- W3080165764 isRetracted "false" @default.
- W3080165764 magId "3080165764" @default.
- W3080165764 workType "book-chapter" @default.