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- W3080255004 endingPage "152543" @default.
- W3080255004 startingPage "152543" @default.
- W3080255004 abstract "The present work, using chromaffin cells in rat adrenal slices (RCCs), aims to describe what type of ionic current alterations induced by zinc underlies their effects reported on synaptic transmission. Thus, Zn 2+ blocked calcium channels of RCCs in a time- and concentration-dependent manner with an IC 50 of 391 μM. This blockade was partially reversed upon washout and was greater at more depolarizing holding potentials (i.e. 32 ± 5% at −110 mV, and 43 ± 6% at -50 mV, after 5 min perfusion). In ω-toxins-sensitive calcium channels (N-, P- and Q-types), Zn 2+ caused a lower blockade of I Ca , 33.3%, than in ω-toxins-resistant ones (L-type, 55.3%; and R-type, 90%). This compound inhibited calcium current at all test potentials and shows a shift of the I-V curve to more depolarized values of about 10 mV. The sodium current was not blocked by acute application of high Zn 2+ concentrations. Voltage-dependent potassium current was marginally affected by high Zn 2+ concentrations showing no concentration-dependence. Nevertheless, calcium- and voltage-dependent potassium current was drastically depressed in a dose-dependent manner, with an IC 50 of 453 μM. This blockade was related to the prevention of Ca 2+ influx through voltage-dependent calcium channels coupled to BK channels. Under current-clamp conditions, RCCs exhibit a resting potential of -50.7 mV, firing spontaneous APs (1–2 spikes/s) generated by the opening of Na + and Ca 2+ -channels, and terminated by the activation of voltage and Ca 2+ -activated K + -channels (BK). We found that the blockade of these ionic currents by Zn 2+ led to a drastic alteration of cellular excitability with a depolarization of the membrane potential, the slowdown and broadening of the APs and the severe reduction of the after hyperpolarization (AHP) which led to a decrease in the APs firing frequency. Taken together, these results point to a neurotoxic action evoked by zinc that is associated with changes to cellular excitability by blocking the ionic currents responsible for both the neurotransmitter release and the action potentials firing." @default.
- W3080255004 created "2020-09-01" @default.
- W3080255004 creator A5024133387 @default.
- W3080255004 creator A5091046880 @default.
- W3080255004 date "2020-11-01" @default.
- W3080255004 modified "2023-09-24" @default.
- W3080255004 title "Micromolar concentrations of Zn2+ depress cellular excitability through a blockade of calcium current in rat adrenal slices" @default.
- W3080255004 cites W1499097678 @default.
- W3080255004 cites W1503147579 @default.
- W3080255004 cites W157666778 @default.
- W3080255004 cites W1616090194 @default.
- W3080255004 cites W1752986334 @default.
- W3080255004 cites W1803630514 @default.
- W3080255004 cites W1817392531 @default.
- W3080255004 cites W1882262859 @default.
- W3080255004 cites W1882452859 @default.
- W3080255004 cites W1937177072 @default.
- W3080255004 cites W1956314911 @default.
- W3080255004 cites W1966548950 @default.
- W3080255004 cites W1966613757 @default.
- W3080255004 cites W1969543864 @default.
- W3080255004 cites W1969731990 @default.
- W3080255004 cites W1971376607 @default.
- W3080255004 cites W1975767206 @default.
- W3080255004 cites W1980325410 @default.
- W3080255004 cites W1980889265 @default.
- W3080255004 cites W1981471614 @default.
- W3080255004 cites W1983375601 @default.
- W3080255004 cites W1984513006 @default.
- W3080255004 cites W1985940938 @default.
- W3080255004 cites W1988144732 @default.
- W3080255004 cites W1989576514 @default.
- W3080255004 cites W1990057321 @default.
- W3080255004 cites W1990557260 @default.
- W3080255004 cites W1990697514 @default.
- W3080255004 cites W1991647860 @default.
- W3080255004 cites W1991890788 @default.
- W3080255004 cites W1992661805 @default.
- W3080255004 cites W1993097538 @default.
- W3080255004 cites W1993876580 @default.
- W3080255004 cites W1999772445 @default.
- W3080255004 cites W2000299259 @default.
- W3080255004 cites W2004007082 @default.
- W3080255004 cites W2006686027 @default.
- W3080255004 cites W2006839087 @default.
- W3080255004 cites W2009909606 @default.
- W3080255004 cites W2013986174 @default.
- W3080255004 cites W2015262462 @default.
- W3080255004 cites W2016489565 @default.
- W3080255004 cites W2016979211 @default.
- W3080255004 cites W2017193677 @default.
- W3080255004 cites W2017597839 @default.
- W3080255004 cites W2018194022 @default.
- W3080255004 cites W2020265449 @default.
- W3080255004 cites W2020682145 @default.
- W3080255004 cites W2021478538 @default.
- W3080255004 cites W2023856103 @default.
- W3080255004 cites W2024105385 @default.
- W3080255004 cites W2025392991 @default.
- W3080255004 cites W2037092705 @default.
- W3080255004 cites W2038937950 @default.
- W3080255004 cites W2039859631 @default.
- W3080255004 cites W2040066177 @default.
- W3080255004 cites W2043930136 @default.
- W3080255004 cites W2044981704 @default.
- W3080255004 cites W2045066127 @default.
- W3080255004 cites W2046423382 @default.
- W3080255004 cites W2047735961 @default.
- W3080255004 cites W2049228395 @default.
- W3080255004 cites W2049942136 @default.
- W3080255004 cites W2054119742 @default.
- W3080255004 cites W2054406771 @default.
- W3080255004 cites W2060086459 @default.
- W3080255004 cites W2063034379 @default.
- W3080255004 cites W2065551144 @default.
- W3080255004 cites W2066824053 @default.
- W3080255004 cites W2067995523 @default.
- W3080255004 cites W2069079626 @default.
- W3080255004 cites W2069473322 @default.
- W3080255004 cites W2071213192 @default.
- W3080255004 cites W2072926617 @default.
- W3080255004 cites W2074875891 @default.
- W3080255004 cites W2077355992 @default.
- W3080255004 cites W2077776173 @default.
- W3080255004 cites W2079354362 @default.
- W3080255004 cites W2079517507 @default.
- W3080255004 cites W2082469108 @default.
- W3080255004 cites W2083500052 @default.
- W3080255004 cites W2084174305 @default.
- W3080255004 cites W2084965566 @default.
- W3080255004 cites W2086421842 @default.
- W3080255004 cites W2089463776 @default.
- W3080255004 cites W2095640158 @default.
- W3080255004 cites W2096458210 @default.
- W3080255004 cites W2098383174 @default.
- W3080255004 cites W2101662524 @default.
- W3080255004 cites W2101672833 @default.
- W3080255004 cites W2103317206 @default.