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• W3080307029 endingPage "12677" @default.
• W3080307029 startingPage "12670" @default.
• W3080307029 abstract "We report a series of colorable zeolitic imidazolate framework (ZIF)-based nanomaterials prepared by encapsulating starches (amylopectin, dextrin, or amylose) or tannic acid in the frameworks of ZIFs and first applied them in colorimetric assay of microRNA/DNA by adding I2/KI or FeCl3 solutions as chromogenic reagents. We found that iodine molecules can lead to rapid degradation of the ZIF-8 framework, while ZIF-90 remains stable. Therefore, ZIF-90 was selected for encapsulating the starches or tannic acid, and then assembled with polyethylenimine (PEI) and aptamers of microRNA/DNA. After interacting with the target microRNA/DNA, the aptamers (Ap) move away from the surface of the prepared Ap–starch@ZIF-90 or Ap–tan@ZIF-90, and the I2/KI or FeCl3 solution is added into the system to interact the starches (amylopectin, dextrin, or amylose) or tannic acid to generate different colors. According to the absorbance spectra, good linear correlations between the logarithm of absorbance intensity and the concentration of microRNA (1–180 nM) can be observed, and the naked eye can distinguish the change from ∼60 to ∼180 nM with a concentration gradient of 20 nM. A similar colorimetric assay ability for pathogenic bacteria can also be realized by detecting the gene fragments IS200 and eaeA. The detection limits can be potentially optimized by changing the amount of adsorbed PEI and aptamers on the surface of Ap–starch@ZIF-90 (or Ap–tan@ZIF-90) nanoparticles. This method could be a promising alternative for simple and cost-effective assay of microRNA/DNA." @default.
• W3080307029 created "2020-09-01" @default.
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• W3080307029 date "2020-08-25" @default.
• W3080307029 modified "2023-09-24" @default.
• W3080307029 title "Colorable Zeolitic Imidazolate Frameworks for Colorimetric Detection of Biomolecules" @default.
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• W3080307029 doi "https://doi.org/10.1021/acs.analchem.0c02895" @default.
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• W3080307029 hasPublicationYear "2020" @default.
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