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- W3080475095 abstract "Immunocytochemistry can be instrumental in assessing the spatial distribution and relative levels of epigenetic modifications. Although conventional immunostaining has been utilized for the detection of 5-methylcytosine (5mC) in animal cells and tissues for several decades, the sensitivity of techniques based on the use of fluorophore-conjugated secondary antibodies is not always sufficient for studying DNA modifications that are less abundant in DNA compared with 5mC. Here we describe a protocol for sensitive immunocytochemistry that utilizes peroxidase-conjugated secondary antibodies coupled with catalyzed reporter deposition and allows for detection of low-abundance noncanonical bases (e.g., 5-carboxylcytosine, 5caC, 5-formylcytosine, 5fC, 5-hydroxymethyluracil, 5hmU) in mammalian DNA. This method can be employed for evaluation of the levels and nuclear distribution of DNA modifications and permits their colocalization with protein markers in animal cells." @default.
- W3080475095 created "2020-09-01" @default.
- W3080475095 creator A5012978930 @default.
- W3080475095 creator A5058939121 @default.
- W3080475095 date "2020-08-22" @default.
- W3080475095 modified "2023-09-26" @default.
- W3080475095 title "Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based Immunocytochemistry" @default.
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- W3080475095 doi "https://doi.org/10.1007/978-1-0716-0876-0_14" @default.
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- W3080475095 hasPublicationYear "2020" @default.
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