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- W3080693684 abstract "Abstract Evaluation of diagnostic capability of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to classify differentially expressed bio-molecular markers in S. typhi infected and healthy freeze-dried sera samples. Using 25 typhoid infected and 25 healthy freeze-dried sera samples, ATR-FTIR spectra were recorded. For evaluating the sample discrimination amid selected wave number regions of FTIR spectra; chemometric data classification algorithms i.e. principal component analysis (PCA), partial least square regression (PLSR) and hierarchical cluster analysis (HCA) were employed. PCA scatter plot showed 90% variation between the S. typhi infected and healthy freeze-dried sera samples. Infection signs were successfully predicted using PLSR model with R2 = 0.9757. HCA dendogram yielded sensitivity and specificity 100% and 96% respectively. ATR-FTIR spectroscopy when coupled with chemometric analysis holds significant potential for biomolecular decimation between S. typhi infected and healthy; freeze-dried sera samples." @default.
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- W3080693684 date "2020-11-01" @default.
- W3080693684 modified "2023-09-24" @default.
- W3080693684 title "Use of ATR-FTIR for detection ofSalmonella typhiinfection in human blood sera" @default.
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- W3080693684 doi "https://doi.org/10.1016/j.infrared.2020.103473" @default.
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