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- W3080853819 abstract "Abstract Recently it has been demonstrated that single-particle cryogenic electron microscopy (cryo-EM) at 200 keV is capable of determining protein structures, including those smaller than 100 kDa, at sub-3.0 Å resolutions, without using significant defocus or a phase plate. However, the majority of near-atomic resolution cryo-EM structures has been determined using 300 keV. Consequently, many typical parameter settings for the cryo-EM computational image processing steps, especially those associated with the contrast transfer function, are based on the accumulated experience of 300 kV cryo-EM. We have therefore revised these parameters, established theoretical bases for criteria to find an optimal mask diameter and box size for a given dataset irrespective of acceleration voltage or protein size, and proposed a protocol. Considering the defocus distributions of the datasets, merely optimizing the mask diameters and box sizes yielded meaningful resolution improvements for the reconstruction of < 200 kDa proteins using 200 kV cryo-EM." @default.
- W3080853819 created "2020-09-01" @default.
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- W3080853819 date "2020-08-24" @default.
- W3080853819 modified "2023-10-10" @default.
- W3080853819 title "Size matters: optimal mask diameter and box size for single-particle cryogenic electron microscopy" @default.
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- W3080853819 doi "https://doi.org/10.1101/2020.08.23.263707" @default.
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