FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3080879462> ?p ?o ?g. }

• W3080879462 endingPage "1945" @default.
• W3080879462 startingPage "1933" @default.
• W3080879462 abstract "BACKGROUND BRCA2 gene, located at chromosome 13q12.3, frequently is altered in familial types of cancer in which a “double-hit” inactivation model seems to occur. In contrast, in sporadic forms of cancer there is frequent absence of a second event (point mutations) suggesting that allelic imbalance at the BRCA2 locus may be associated with a “gene dosage effect” of BRCA2 function. Little is known about BRCA2 allelic alterations in nonsmall cell lung carcinomas (NSCLCs). Furthermore, recent studies suggest that BRCA2 and p53 participate in a common pathway involved in DNA damage repair. In view of this putative link, the authors investigated in a series of 63 NSCLCs: 1) the allelic imbalance (AIm) at the D13S171 (BRCA2) locus, 2) the possible relation with tumor kinetics (proliferation [PI] and apoptotic indices [AI]) and chromosomal instability (aneuploidy) of the carcinomas, and 3) the mutual impact of D13S171 AIm and p53 altered status on the above-mentioned parameters. METHODS Allelic status of the BRCA2 region was examined in a series of 63 NSCLCs, by using the polymorphic marker D13S171, which is located in the center of it. Most information regarding the status of p53 at the immunohistochemical and genetic levels was obtained from a previous analysis. Tumor kinetic parameters (proliferation and apoptotic indices) were determined using Ki-67 immunohistochemical analysis and Tdt-mediated dUTP nick end labeling assay, respectively. Chromosomal instability (aneuploidy) was assessed by measuring nuclear DNA ploidy with an image analysis system. RESULTS Allelic imbalance at D13S171(BRCA2) was observed in 70% of the informative cases (H: heterozygous) with a rather high frequency of occurrence (50%) in Stage I disease, suggesting a possible early involvement in the development of NSCLCs. Although no association was found among loss of heterozygosity (LOH) at D13S171, kinetic parameters and ploidy status of the tumors and concurrent alterations in BRCA2 and p53 (BRCA2[LOH]/p53[P]), which was the most frequent profile (37.2%), had the highest growth index (PI/AI mean value ratio) that differed significantly only from the BRCA2(LOH)/p53(N) pattern (P = 0.027). This difference was attributed to the high AI of the BRCA2(LOH)/p53(N) pattern (P < 0.001), whereas PI was similar among all BRCA2/p53 profiles. Also the “full abnormal pattern” was associated with aneuploidy, whereas the BRCA2(LOH)/p53(N) profile was mainly diploid. When these indicators and conventional prognostic ones were examined for effect on patient survival, only stage and lymph node status showed a significant correlation, whereas LOH at D13S171 (BRCA2), p53 abnormalities, proliferative and apoptotic indices, ploidy status, smoking history, and histology and combinations of LOH and p53 abnormalities failed to show significant correlation with survival. CONCLUSIONS These findings suggest that in BRCA2(LOH) NSCLCs the status of p53 (wild type or mutant) represents a decisive determinant of tumor growth and chromosomal instability. Nevertheless, a possible synergistic effect from loss of D13S171 region with p53 abnormalities cannot be excluded because the BRCA2(LOH)/p53(P) profile compared with the BRCA2(H)/p53(P) one had a higher PI/AI mean value ratio (31.05 vs. 22.97), although it was not statistically significant. However, we cannot exclude the possibility that LOH at D13S171 reflects deletion of other putative tumor suppressor gene(s) in the proximity of BRCA2. In this respect, more studies are needed to understand the involvement of BRCA2 region alterations in nonsmall cell lung carcinogenesis. Cancer 2000;89:1933–45. © 2000 American Cancer Society." @default.
• W3080879462 created "2020-09-01" @default.
• W3080879462 creator A5016122708 @default.
• W3080879462 creator A5019722388 @default.
• W3080879462 creator A5020765581 @default.
• W3080879462 creator A5022001501 @default.
• W3080879462 creator A5025738282 @default.
• W3080879462 creator A5032902440 @default.
• W3080879462 creator A5034359692 @default.
• W3080879462 creator A5042531154 @default.
• W3080879462 creator A5050104326 @default.
• W3080879462 creator A5084504188 @default.
• W3080879462 date "2000-10-30" @default.
• W3080879462 modified "2023-09-27" @default.
• W3080879462 title "Association of allelic imbalance at locus D13S171 (BRCA2) and p53 alterations with tumor kinetics and chromosomal instability (aneuploidy) in nonsmall cell lung carcinoma" @default.
• W3080879462 cites W1544851750 @default.
• W3080879462 cites W1865181509 @default.
• W3080879462 cites W189821932 @default.
• W3080879462 cites W1917937658 @default.
• W3080879462 cites W1967080458 @default.
• W3080879462 cites W1972087107 @default.
• W3080879462 cites W1980191680 @default.
• W3080879462 cites W1991586667 @default.
• W3080879462 cites W1997388033 @default.
• W3080879462 cites W2000437208 @default.
• W3080879462 cites W2002555966 @default.
• W3080879462 cites W2004967361 @default.
• W3080879462 cites W2005511378 @default.
• W3080879462 cites W2019205168 @default.
• W3080879462 cites W2024342001 @default.
• W3080879462 cites W2024986660 @default.
• W3080879462 cites W2026616574 @default.
• W3080879462 cites W2037954444 @default.
• W3080879462 cites W2038463413 @default.
• W3080879462 cites W2047273109 @default.
• W3080879462 cites W2054462805 @default.
• W3080879462 cites W2061011566 @default.
• W3080879462 cites W2067028981 @default.
• W3080879462 cites W2069125713 @default.
• W3080879462 cites W2069881315 @default.
• W3080879462 cites W2070607408 @default.
• W3080879462 cites W2079388655 @default.
• W3080879462 cites W2085579435 @default.
• W3080879462 cites W2087404852 @default.
• W3080879462 cites W2124726835 @default.
• W3080879462 cites W2129142610 @default.
• W3080879462 cites W2135309140 @default.
• W3080879462 cites W2138302361 @default.
• W3080879462 cites W2153926167 @default.
• W3080879462 cites W2161260766 @default.
• W3080879462 cites W2169859003 @default.
• W3080879462 cites W2324462075 @default.
• W3080879462 cites W2327319275 @default.
• W3080879462 cites W2331734181 @default.
• W3080879462 cites W2332724067 @default.
• W3080879462 doi "https://doi.org/10.1002/1097-0142(20001101)89:9<1933::aid-cncr9>3.0.co;2-d" @default.
• W3080879462 hasPublicationYear "2000" @default.
• W3080879462 type Work @default.
• W3080879462 sameAs 3080879462 @default.
• W3080879462 citedByCount "0" @default.
• W3080879462 crossrefType "journal-article" @default.
• W3080879462 hasAuthorship W3080879462A5016122708 @default.
• W3080879462 hasAuthorship W3080879462A5019722388 @default.
• W3080879462 hasAuthorship W3080879462A5020765581 @default.
• W3080879462 hasAuthorship W3080879462A5022001501 @default.
• W3080879462 hasAuthorship W3080879462A5025738282 @default.
• W3080879462 hasAuthorship W3080879462A5032902440 @default.
• W3080879462 hasAuthorship W3080879462A5034359692 @default.
• W3080879462 hasAuthorship W3080879462A5042531154 @default.
• W3080879462 hasAuthorship W3080879462A5050104326 @default.
• W3080879462 hasAuthorship W3080879462A5084504188 @default.
• W3080879462 hasBestOaLocation W30808794621 @default.
• W3080879462 hasConcept C104317684 @default.
• W3080879462 hasConcept C121608353 @default.
• W3080879462 hasConcept C140752955 @default.
• W3080879462 hasConcept C142724271 @default.
• W3080879462 hasConcept C143425029 @default.
• W3080879462 hasConcept C143589142 @default.
• W3080879462 hasConcept C178169997 @default.
• W3080879462 hasConcept C180754005 @default.
• W3080879462 hasConcept C2776256026 @default.
• W3080879462 hasConcept C2778264664 @default.
• W3080879462 hasConcept C2779672484 @default.
• W3080879462 hasConcept C30481170 @default.
• W3080879462 hasConcept C502942594 @default.
• W3080879462 hasConcept C54355233 @default.
• W3080879462 hasConcept C552990157 @default.
• W3080879462 hasConcept C555283112 @default.
• W3080879462 hasConcept C71924100 @default.
• W3080879462 hasConcept C84597430 @default.
• W3080879462 hasConcept C86803240 @default.
• W3080879462 hasConceptScore W3080879462C104317684 @default.
• W3080879462 hasConceptScore W3080879462C121608353 @default.
• W3080879462 hasConceptScore W3080879462C140752955 @default.
• W3080879462 hasConceptScore W3080879462C142724271 @default.
• W3080879462 hasConceptScore W3080879462C143425029 @default.
• W3080879462 hasConceptScore W3080879462C143589142 @default.
• W3080879462 hasConceptScore W3080879462C178169997 @default.

• next