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- W308091982 abstract "Our earlier experiments were further continued with systems containing gel particles in oleic acid. In these cases the fermentation culture media were inside the gel beads only. To build such systems we prepared complex sterile solution containing sodium-alginate, yeast cells, their additives and various amount of glucose (100-400 g l -1 ). These solutions were dropped in Ca-chloride precipitating solutions. The gel beads were suspended directly in oleic acid. The glucose was uptaken by yeast cells quantitatively during 24-72 h. The uptake rate of glucose is dependent on the concentration of glucose. It was similar to the free cell fermentation: in cases of higher concentrations of glucose the glucose uptake in the second period was stopped. In accordance with changes of the concentration of glucose the concentration of ethanol increased rapidly in the first day of fermentation and thereafter slowed down. We supposed that by co-immobilization of lipase in the same gel particles the inhibitory effect of produced ethanol would be solved. Using of lipase we can transform ethanol to ethyl-oleate, which is soluble in oleic acid. According to our data, at least 4 U ml -1 lipase is needed to increase ethanol production significantly Summarising we can conclude that in our system the maximum ethanol production can be achieved using 100 g l -1 glucose and 4 U ml -1 lipase for 48 h. In this system 50 g l -1 ethanol can be produced by 48 h fermentation and practically a steady state concentration of ethanol is maintained after it." @default.
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- W308091982 date "1999-03-01" @default.
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- W308091982 title "Extractive fermentation of ethanol using alginate gel co-entrapped yeast cells (Saccharomyces Bayanus) and lipase enzyme" @default.
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