FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3081025830> ?p ?o ?g. }

• W3081025830 endingPage "1122" @default.
• W3081025830 startingPage "1115" @default.
• W3081025830 abstract "To analyze the significance of various abnormal signal patterns appreared in CML and B-ALL patients by using BCR/ABL/ASS1 tricolor dual-fusion probe, and to explore its application value in detecting BCR/ABL fusion gene and ASS1 gene deletion.50 newly diagnosed CML patients and 50 newly diagnosed B-ALL patients were detected by fluorescence in situ hybridization (FISH) with BCR/ABL/ASS1 tricolor dual-fusion probe. Meanwhile, karyotype analysis was performed on all the patients using the 24 hours short-term culture and R-banding.Among the 50 CML patients, Ph+ was found in 49 cases, 5 normal interphase karyotype was observed in 1 case. FISH detection showed that BCR/ABL fusion gene existed in all patients (100%), while the positive signal pathway showed that 1R1G2B2F was observed in 39 cases (78%), 2R1G2B1F in 2 cases (4%) and 1R1G2B1F in 6 cases (12%), simultaneous existence of 1R1G1B1F and 1R1G2B3F in 1 case (2%), 2R1G1B1F in 1 case (2%) 1R1G3B3F in 1 case (2%). FISH detection also showed that the karyotype of 6 case at ASS1 gene deletion (1R1G1B1F) all were simple t (9; 22) translocation, and other abnormalities not were observed. Among 50 cases of B-ALL, Ph+ was found in 13 cases, the numerical aberration and structural aberration of non t (9; 22) in 16 cases, normal karyotype in 20 cases, absence of mitotic phase in 1 case. FISH detection showed that 16 cases (32%) had BCR/ABL fusion gene including 13 cases (26%) of 1R1G2B2F, 1 case (2%) of stimultaneous exitance of 1R1G2B2F and 1R1G3B3F 1 case (2%) of 2R1G1B1F, 1 case (2%) of 1R1G3B2F. FISH detection also showed that 3 cases had BCR/ABL fusion gene, including 1 case with ASS1 gene deletion (2R1G1B1F), 1 case with classical t (9; 22) translocation (1R1G2B2F) and 1 case with BCR/ABL fusion gene and increase of ASS1 gene copy (1R1G3B3F).Tricolor dual-fusion FISH probe for detecting BCR/ABL fusion gene and ASS1 gene deletion is simple, rapid, sensitive and stable. It can detect various forms of molecular fusion and avoid the false positive results due to coincidental overlap of signals generated by D-FISH probe and ES-FISH probe. In addition, this detection method not only can directly observe the presence or absence of ASS1 gene deletion, but also improve the reliability of the positive results of newly diagnosed BCR/ABL fusion gene and accuracy of monitoring results of minimal residual disease for the subsequent visit.应用三色双融合探针检测BCR-ABL融合基因及ASS1基因缺失.分析BCR/ABL/ASS1三色双融合探针在CML和B-ALL所出现的各种异常信号模式的意义，并探讨其在检测BCR/ABL融合基因及ASS1基因缺失中的应用价值.分别对50例初诊CML患者和50例初诊B-ALL患者采用BCR/ABL/ASS1三色双融合探针进行荧光原位杂交（FISH）检测，同时对所有病例应用24 h短期培养法R显带后进行染色体核型分析.50例CML患者中，49例Ph+，余1例可见5个正常中期核型；通过 FISH发现，所有患者（100%）均存在BCR/ABL融合基因，阳性信号特征分别为1R1G2B2F 39例（78%）、 2R1G2B1F 2例（4%）、1R1G1B1F 6例（12%）、同时伴有1R1G2B2F和1R1G2B3F 2例（4%）、2R2G2B1F 1例（2%）。伴有ASS1基因缺失（1R1G1B1F）的6例患者染色体核型均为单纯t（9；22）易位，无其他异常。50例B-ALL患者中，Ph+ 13例，数目畸变及非t（9；22）的结构畸变16例，正常核型20例，无分裂相1例；经FISH检出16例（32%）具有BCR/ABL融合基因，分别为1R1G2B2F 13例（26%）、同时伴有1R1G2B2F和1R1G2B3F 1例（2%）、 2R1G1B1F 1例（2%）、1R1G3B3F 1例（2%），FISH额外检出的3例BCR/ABL融合基因阳性包括1例伴有ASS1基因缺失（2R1G1B1F）、1例经典型t（9；22）易位（1R1G2B2F）和1例BCR/ABL融合基因及ASS1基因拷贝数的增加（1R1G3B3F）.应用三色双融合FISH探针检测BCR/ABL融合基因及ASS1基因缺失，具有简单、快速、灵敏、稳定的特点，能够检测多种形式的分子融合，可很好地避免D-FISH探针及ES-FISH探针因信号随机重叠导致的假阳性结果。该检测方法不但可以直接观察有无ASS1基因的缺失，而且还能提高初诊BCR/ABL融合基因阳性结果的可靠性及复诊监测微小残留病结果的准确性." @default.
• W3081025830 created "2020-09-01" @default.
• W3081025830 creator A5002990257 @default.
• W3081025830 creator A5027220663 @default.
• W3081025830 creator A5033822228 @default.
• W3081025830 creator A5056530877 @default.
• W3081025830 creator A5061082958 @default.
• W3081025830 creator A5062642238 @default.
• W3081025830 creator A5089850088 @default.
• W3081025830 date "2020-08-01" @default.
• W3081025830 modified "2023-10-16" @default.
• W3081025830 title "[Detection of BCR/ABL Fusion Gene and ASS1 Gene Deletion by Using Tricolor Dual-fusion Probe]." @default.
• W3081025830 doi "https://doi.org/10.19746/j.cnki.issn.1009-2137.2020.04.006" @default.
• W3081025830 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32798385" @default.
• W3081025830 hasPublicationYear "2020" @default.
• W3081025830 type Work @default.
• W3081025830 sameAs 3081025830 @default.
• W3081025830 citedByCount "0" @default.
• W3081025830 crossrefType "journal-article" @default.
• W3081025830 hasAuthorship W3081025830A5002990257 @default.
• W3081025830 hasAuthorship W3081025830A5027220663 @default.
• W3081025830 hasAuthorship W3081025830A5033822228 @default.
• W3081025830 hasAuthorship W3081025830A5056530877 @default.
• W3081025830 hasAuthorship W3081025830A5061082958 @default.
• W3081025830 hasAuthorship W3081025830A5062642238 @default.
• W3081025830 hasAuthorship W3081025830A5089850088 @default.
• W3081025830 hasConcept C104317684 @default.
• W3081025830 hasConcept C111829193 @default.
• W3081025830 hasConcept C125418893 @default.
• W3081025830 hasConcept C138626823 @default.
• W3081025830 hasConcept C153911025 @default.
• W3081025830 hasConcept C170493617 @default.
• W3081025830 hasConcept C2777542201 @default.
• W3081025830 hasConcept C30481170 @default.
• W3081025830 hasConcept C42362537 @default.
• W3081025830 hasConcept C43907098 @default.
• W3081025830 hasConcept C53226629 @default.
• W3081025830 hasConcept C54355233 @default.
• W3081025830 hasConcept C86803240 @default.
• W3081025830 hasConceptScore W3081025830C104317684 @default.
• W3081025830 hasConceptScore W3081025830C111829193 @default.
• W3081025830 hasConceptScore W3081025830C125418893 @default.
• W3081025830 hasConceptScore W3081025830C138626823 @default.
• W3081025830 hasConceptScore W3081025830C153911025 @default.
• W3081025830 hasConceptScore W3081025830C170493617 @default.
• W3081025830 hasConceptScore W3081025830C2777542201 @default.
• W3081025830 hasConceptScore W3081025830C30481170 @default.
• W3081025830 hasConceptScore W3081025830C42362537 @default.
• W3081025830 hasConceptScore W3081025830C43907098 @default.
• W3081025830 hasConceptScore W3081025830C53226629 @default.
• W3081025830 hasConceptScore W3081025830C54355233 @default.
• W3081025830 hasConceptScore W3081025830C86803240 @default.
• W3081025830 hasIssue "4" @default.
• W3081025830 hasLocation W30810258301 @default.
• W3081025830 hasOpenAccess W3081025830 @default.
• W3081025830 hasPrimaryLocation W30810258301 @default.
• W3081025830 hasRelatedWork W190106789 @default.
• W3081025830 hasRelatedWork W1991837960 @default.
• W3081025830 hasRelatedWork W2023339250 @default.
• W3081025830 hasRelatedWork W2076243606 @default.
• W3081025830 hasRelatedWork W2152043134 @default.
• W3081025830 hasRelatedWork W2352528894 @default.
• W3081025830 hasRelatedWork W2367615558 @default.
• W3081025830 hasRelatedWork W2372941326 @default.
• W3081025830 hasRelatedWork W2592232437 @default.
• W3081025830 hasRelatedWork W4234894020 @default.
• W3081025830 hasVolume "28" @default.
• W3081025830 isParatext "false" @default.
• W3081025830 isRetracted "false" @default.
• W3081025830 magId "3081025830" @default.
• W3081025830 workType "article" @default.