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- W3081128894 abstract "Regulation of physiological pH is integral for proper whole body and cellular function, and disruptions in pH homeostasis can be both a cause and effect of disease. In light of this, many methods have been developed to monitor pH in cells and animals. In this study, we report a chemiluminescence resonance energy transfer (CRET) probe Ratio-pHCL-1, composed of an acrylamide 1,2-dioxetane chemiluminescent scaffold with an appended pH-sensitive carbofluorescein fluorophore. The probe provides an accurate measurement of pH between 6.8 and 8.4, making it a viable tool for measuring pH in biological systems. Further, its ratiometric output is independent of confounding variables. Quantification of pH can be accomplished using both common luminescence spectroscopy and advanced optical imaging methods. Using an IVIS Spectrum, pH can be measured through tissue with Ratio-pHCL-1, which is shown in vitro and calibrated in sacrificed mouse models. Intraperitoneal injections of Ratio-pHCL-1 into live mice show high photon outputs and consistent increases in the flux ratio when measured at pH 6, 7, and 8." @default.
- W3081128894 created "2020-09-01" @default.
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- W3081128894 date "2020-08-24" @default.
- W3081128894 modified "2023-10-10" @default.
- W3081128894 title "Ratiometric pH Imaging Using a 1,2-Dioxetane Chemiluminescence Resonance Energy Transfer Sensor in Live Animals" @default.
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- W3081128894 cites W1970756909 @default.
- W3081128894 cites W1971778847 @default.
- W3081128894 cites W1980800538 @default.
- W3081128894 cites W1985565475 @default.
- W3081128894 cites W2001858901 @default.
- W3081128894 cites W2002811168 @default.
- W3081128894 cites W2014071478 @default.
- W3081128894 cites W2018343578 @default.
- W3081128894 cites W2024646526 @default.
- W3081128894 cites W2025688726 @default.
- W3081128894 cites W2030630366 @default.
- W3081128894 cites W2048251162 @default.
- W3081128894 cites W2049061652 @default.
- W3081128894 cites W2054816860 @default.
- W3081128894 cites W2093071815 @default.
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- W3081128894 cites W2108944651 @default.
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- W3081128894 cites W2124479575 @default.
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- W3081128894 cites W2511142927 @default.
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- W3081128894 cites W2538965655 @default.
- W3081128894 cites W2591844450 @default.
- W3081128894 cites W2621478401 @default.
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- W3081128894 cites W2745933126 @default.
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- W3081128894 cites W2804174210 @default.
- W3081128894 cites W2903557147 @default.
- W3081128894 cites W2904616005 @default.
- W3081128894 cites W2906329344 @default.
- W3081128894 cites W2906868418 @default.
- W3081128894 cites W2912606030 @default.
- W3081128894 cites W2940033546 @default.
- W3081128894 cites W2942005671 @default.
- W3081128894 cites W2955953987 @default.
- W3081128894 cites W2970405392 @default.
- W3081128894 cites W2971436900 @default.
- W3081128894 cites W2978318548 @default.
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- W3081128894 cites W2984030372 @default.
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- W3081128894 doi "https://doi.org/10.1021/acssensors.0c01393" @default.
- W3081128894 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32829636" @default.
- W3081128894 hasPublicationYear "2020" @default.
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