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• W3081163661 endingPage "4661" @default.
• W3081163661 startingPage "4643" @default.
• W3081163661 abstract "Key points The Arg271Gln mutation of the glycine receptor (GlyR) causes hereditary hyperekplexia. This mutation dramatically compromises GlyR function; however, the underlying mechanism is not yet known. This study, by employing function and computation methods, proposes that charged residues (including the Arg residue) at the pore extracellular half from each of the five subunits of the homomeric α1 GlyR, create an electrostatic repulsive potential to widen the pore, thereby facilitating channel opening. This mechanism explains how the Arg271Gln mutation, in which the positively charged Arg residue is substituted by the neutral Gln residue, compromises GlyR function. This study furthers our understanding of the biophysical mechanism underlying the Arg271Gln mutation compromising GlyR function. Abstract The R271(19′)Q mutation in the α1 subunit of the glycine receptor (GlyR) chloride channel causes hereditary hyperekplexia. This mutation dramatically compromises channel function; however, the underlying mechanism is not yet known. The R271 residue is located at the extracellular half of the channel pore. In this study, an Arg‐scanning mutagenesis was performed at the pore extracellular half from the 262(10′) to the 272(20′) position on the background of the α1 GlyR carrying the hyperekplexia‐causing mutation R271(19′)Q. It was found that the placement of the Arg residue rescued channel function to an extent inversely correlated with the distance between the residue and the pore central axis (perpendicular to the plane of the lipid bilayer). Accordingly, it was hypothesized that the placed Arg residues from each of the five subunits of the homomeric α1 GlyR create an electrostatic repulsive potential to widen the pore, thereby facilitating channel opening. This hypothesis was quantitatively verified by theoretical computation via exploiting basic laws of electrostatics and thermodynamics, and further supported by more experimental findings that the placement of another positively charged Lys residue or even a negatively charged Asp residue also rescued channel function in the same manner. This study provides a novel mechanism via which charged residues in the pore region facilitate channel gating, not only for the disease‐causing 19′R residue in the GlyR, but also potentially for charged residues in the same region of other ion channels." @default.
• W3081163661 created "2020-09-01" @default.
• W3081163661 creator A5016587132 @default.
• W3081163661 creator A5043422504 @default.
• W3081163661 creator A5052354691 @default.
• W3081163661 date "2020-08-26" @default.
• W3081163661 modified "2023-10-16" @default.
• W3081163661 title "Charged residues at the pore extracellular half of the glycine receptor facilitate channel gating: a potential role played by electrostatic repulsion" @default.
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• W3081163661 doi "https://doi.org/10.1113/jp279288" @default.
• W3081163661 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32844405" @default.
• W3081163661 hasPublicationYear "2020" @default.

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