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• W3081211798 abstract "Abstract Quantifying the genetic heterogeneity of a cell population is essential to understanding of biological systems. We develop a universal method to label individual DNA molecules for single-base-resolution haplotype-resolved quantitative characterization of diverse types of rare variants, with frequency as low as 4 × 10 −5 , using both short- or long-read sequencing platforms. It provides the first quantitative evidence of persistent nonrandom large structural variants and an increase in single-nucleotide variants at the on-target locus following repair of double-strand breaks induced by CRISPR-Cas9 in human embryonic stem cells." @default.
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• W3081211798 date "2020-08-24" @default.
• W3081211798 modified "2023-10-05" @default.
• W3081211798 title "Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs" @default.
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• W3081211798 doi "https://doi.org/10.1186/s13059-020-02143-8" @default.
• W3081211798 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7444080" @default.
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• W3081211798 hasPublicationYear "2020" @default.
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