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- W3081282143 abstract "Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34+ progenitor and more differentiated CD34- fractions from post-natal thymic tissue to study thymopoiesis. CD34+ cells represent <1% of thymic cells, so this protocol uses magnetic- followed by fluorescence-activated cell separation to isolate highly enriched CD34+ cells. For complete details on the use and execution of this protocol, please refer to Le et al. (2020)." @default.
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- W3081282143 date "2020-09-01" @default.
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- W3081282143 title "Processing Human Thymic Tissue for Single Cell RNA-Seq" @default.
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- W3081282143 doi "https://doi.org/10.1016/j.xpro.2020.100090" @default.
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