FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3081592690> ?p ?o ?g. }

• W3081592690 abstract "ABSTRACT Euphausiids (or krill) are important contributors to marine biomass and key players in marine pelagic trophic webs. Euphausiids stomachs represent a specific niche for microbes that participate in the digestion of the host dietary components. To date, methods for the study of the diversity and function of these microorganisms remain complex. Often, bacterial ribosomal sequences obtained from lysates of stomachs are overrepresented by organisms from the surrounding environment. Flow cytometry with cell sorting (FC-CS) have become a powerful technique to study microbial community structure but also for the study of population genomics of gut-associated bacteria, even at a single-cell level. In this study, we used FC-CS and sequencing of the bacterial 16S rRNA gene to study the microorganisms inhabiting the stomach of the Humboldt Current krill, Euphausia mucronata . This approach was complemented with DNA extraction and sequencing from whole lysate stomachs as described for other crustacean species. Non-specific amplification was not retrieved in the polymerase chain reaction (PCR) from cells sorted, opposite to the observed using the DNA from the whole lysate. Sequences obtained from the whole stomach DNA were enriched in picocyanobacteria, meanwhile, sequences retrieved from cells sorted belonged almost exclusively to Balneola sp. of the new phylum, Balneolaeota. This study represents, to our knowledge, the first report of Balneola sp. in the stomach for any organism inhabiting the Humboldt Current System (HCS). Our results suggest that the stomach-associated microbiota can be characterized by FC-CS and sequencing by manual scraping of the stomach coupled with the DNA extraction and sequencing. This work represents a baseline for similar studies of other mesozooplankton groups. The implementation of this technique might complement future studies on host-microbes’ interaction and their implications on the marine pelagic food web." @default.
• W3081592690 created "2020-09-08" @default.
• W3081592690 creator A5001686873 @default.
• W3081592690 creator A5005165604 @default.
• W3081592690 creator A5018581819 @default.
• W3081592690 creator A5052392196 @default.
• W3081592690 creator A5091470928 @default.
• W3081592690 date "2020-08-31" @default.
• W3081592690 modified "2023-10-17" @default.
• W3081592690 title "Flow cytometry with cell sorting and sequencing as a tool for the study of the Humboldt Current krill stomach microbiota" @default.
• W3081592690 cites W1598574080 @default.
• W3081592690 cites W1701869895 @default.
• W3081592690 cites W1724099464 @default.
• W3081592690 cites W1769030150 @default.
• W3081592690 cites W1969042907 @default.
• W3081592690 cites W1970769556 @default.
• W3081592690 cites W1982491165 @default.
• W3081592690 cites W1989912787 @default.
• W3081592690 cites W1991693735 @default.
• W3081592690 cites W2026347365 @default.
• W3081592690 cites W2027871674 @default.
• W3081592690 cites W2028057427 @default.
• W3081592690 cites W2029822387 @default.
• W3081592690 cites W2037560033 @default.
• W3081592690 cites W2038773679 @default.
• W3081592690 cites W2058357169 @default.
• W3081592690 cites W2058744331 @default.
• W3081592690 cites W2081809293 @default.
• W3081592690 cites W2085643274 @default.
• W3081592690 cites W2096367688 @default.
• W3081592690 cites W2111858111 @default.
• W3081592690 cites W2124967403 @default.
• W3081592690 cites W2142648753 @default.
• W3081592690 cites W2144353611 @default.
• W3081592690 cites W2153601235 @default.
• W3081592690 cites W2154999768 @default.
• W3081592690 cites W2155101814 @default.
• W3081592690 cites W2157281339 @default.
• W3081592690 cites W2196068809 @default.
• W3081592690 cites W2326136856 @default.
• W3081592690 cites W2326366979 @default.
• W3081592690 cites W2439174287 @default.
• W3081592690 cites W2462736029 @default.
• W3081592690 cites W2476286190 @default.
• W3081592690 cites W2495112139 @default.
• W3081592690 cites W2517204038 @default.
• W3081592690 cites W2546696368 @default.
• W3081592690 cites W2611198866 @default.
• W3081592690 cites W2789547748 @default.
• W3081592690 cites W2909578929 @default.
• W3081592690 doi "https://doi.org/10.1101/2020.08.31.275677" @default.
• W3081592690 hasPublicationYear "2020" @default.
• W3081592690 type Work @default.
• W3081592690 sameAs 3081592690 @default.
• W3081592690 citedByCount "0" @default.
• W3081592690 crossrefType "posted-content" @default.
• W3081592690 hasAuthorship W3081592690A5001686873 @default.
• W3081592690 hasAuthorship W3081592690A5005165604 @default.
• W3081592690 hasAuthorship W3081592690A5018581819 @default.
• W3081592690 hasAuthorship W3081592690A5052392196 @default.
• W3081592690 hasAuthorship W3081592690A5091470928 @default.
• W3081592690 hasBestOaLocation W30815926901 @default.
• W3081592690 hasConcept C104317684 @default.
• W3081592690 hasConcept C144024400 @default.
• W3081592690 hasConcept C149923435 @default.
• W3081592690 hasConcept C15151743 @default.
• W3081592690 hasConcept C175605896 @default.
• W3081592690 hasConcept C17757408 @default.
• W3081592690 hasConcept C18903297 @default.
• W3081592690 hasConcept C2775881529 @default.
• W3081592690 hasConcept C2777492549 @default.
• W3081592690 hasConcept C2908647359 @default.
• W3081592690 hasConcept C42062724 @default.
• W3081592690 hasConcept C51679486 @default.
• W3081592690 hasConcept C523546767 @default.
• W3081592690 hasConcept C54355233 @default.
• W3081592690 hasConcept C552990157 @default.
• W3081592690 hasConcept C67905577 @default.
• W3081592690 hasConcept C86803240 @default.
• W3081592690 hasConcept C89423630 @default.
• W3081592690 hasConceptScore W3081592690C104317684 @default.
• W3081592690 hasConceptScore W3081592690C144024400 @default.
• W3081592690 hasConceptScore W3081592690C149923435 @default.
• W3081592690 hasConceptScore W3081592690C15151743 @default.
• W3081592690 hasConceptScore W3081592690C175605896 @default.
• W3081592690 hasConceptScore W3081592690C17757408 @default.
• W3081592690 hasConceptScore W3081592690C18903297 @default.
• W3081592690 hasConceptScore W3081592690C2775881529 @default.
• W3081592690 hasConceptScore W3081592690C2777492549 @default.
• W3081592690 hasConceptScore W3081592690C2908647359 @default.
• W3081592690 hasConceptScore W3081592690C42062724 @default.
• W3081592690 hasConceptScore W3081592690C51679486 @default.
• W3081592690 hasConceptScore W3081592690C523546767 @default.
• W3081592690 hasConceptScore W3081592690C54355233 @default.
• W3081592690 hasConceptScore W3081592690C552990157 @default.
• W3081592690 hasConceptScore W3081592690C67905577 @default.
• W3081592690 hasConceptScore W3081592690C86803240 @default.
• W3081592690 hasConceptScore W3081592690C89423630 @default.
• W3081592690 hasLocation W30815926901 @default.
• W3081592690 hasOpenAccess W3081592690 @default.

• next