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- W3081704319 abstract "This work presents the development and application of an ultrasensitive photoelectrochemical biosensor based on copper (I) sulfide (Cu2S) and horseradish peroxidase enzyme (HRP) immobilized on a fluorine-doped tin oxide modified glass slide (FTO) for the determination of 1,4-dihydroxybenzene (DHB). The Cu2S/FTO photoelectrochemical platform was characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). The electrochemical properties of the proposed photoelectrochemical biosensor (HRP-Cu2S/FTO) were evaluated by electrochemical impedance spectroscopy (EIS) and amperometry. The photocurrent of the photoelectrochemical platform was amperometrically monitored, using a commercial 20 W white LED light bulb as the source of light. After optimization of experimental parameters, the biosensor had shown good responses for detection of DHB, in the presence of 50 µmol L−1 of hydrogen peroxide, exhibiting a wide linear range for DHB concentrations between 10 nmol L−1 up to 1 mmol L−1 (r2 = 0.998), with a detection limit of 4.0 nmol L−1. The selectivity of the photoelectrochemical biosensor was also evaluated and the results demonstrated that the proposed biosensor presents a good selectivity for DHB detection. The HRP-Cu2S/FTO photoelectrochemical biosensor was applied in skin cream samples presenting recovery percentages for the analyte in the samples between 99.89 and 100.70%, suggesting a good accuracy for the proposed method." @default.
- W3081704319 created "2020-09-08" @default.
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- W3081704319 date "2020-12-01" @default.
- W3081704319 modified "2023-10-06" @default.
- W3081704319 title "Photoelectrochemical biosensor for 1,4-dihydroxybenzene based on copper sulfide and horseradish peroxidase enzyme: Application in skin cream samples" @default.
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- W3081704319 doi "https://doi.org/10.1016/j.microc.2020.105487" @default.
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