FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3082013852> ?p ?o ?g. }

• W3082013852 endingPage "106" @default.
• W3082013852 startingPage "94" @default.
• W3082013852 abstract "Enhanced intracellular survival (Eis) proteins belonging to the superfamily of the GCN5-related N-acetyltransferases play important functions in mycobacterial pathogenesis. In Mycobacterium tuberculosis, Eis enhances the intracellular survival of the bacilli in macrophages by modulating the host immune response and is capable to chemically modify and inactivate aminoglycosides. In nontuberculous mycobacteria (NTM), Eis shares similar functions. However, Mycobacterium abscessus, a multidrug resistant NTM, possesses two functionally distinct Eis homologues, Eis1Mab and Eis2Mab . While Eis2Mab participates in virulence and aminoglycosides resistance, this is not the case for Eis1Mab, whose exact biological function remains to be determined. Herein, we show that overexpression of Eis1Mab in M. abscessus fails to induce resistance to aminoglycosides. To clarify why Eis1Mab is unable to modify this class of antibiotics, we solved its crystal structure bound to its cofactor, acetyl-CoA. The structure revealed that Eis1Mab has a typical homohexameric Eis-like organization. The structural analysis supported by biochemical approaches demonstrated that while Eis1Mab can acetylate small substrates, its active site is too narrow to accommodate aminoglycosides. Comparison with other Eis structures showed that an extended loop between strands 9 and 10 is blocking the access of large substrates to the active site and movement of helices 4 and 5 reduces the volume of the substrate-binding pocket to these compounds in Eis1Mab . Overall, this study underscores the molecular determinants explaining functional differences between Eis1Mab and Eis2Mab, especially those inherent to their capacity to modify aminoglycosides." @default.
• W3082013852 created "2020-09-08" @default.
• W3082013852 creator A5056305582 @default.
• W3082013852 creator A5058577900 @default.
• W3082013852 creator A5086461817 @default.
• W3082013852 date "2020-09-12" @default.
• W3082013852 modified "2023-10-14" @default.
• W3082013852 title "Structural analysis of the <i>N</i>‐acetyltransferase Eis1 from <i>Mycobacterium abscessus</i> reveals the molecular determinants of its incapacity to modify aminoglycosides" @default.
• W3082013852 cites W1966572391 @default.
• W3082013852 cites W1969719733 @default.
• W3082013852 cites W1973456026 @default.
• W3082013852 cites W1980465469 @default.
• W3082013852 cites W2018937854 @default.
• W3082013852 cites W2030517878 @default.
• W3082013852 cites W2033672945 @default.
• W3082013852 cites W2055473780 @default.
• W3082013852 cites W2063475394 @default.
• W3082013852 cites W2078290231 @default.
• W3082013852 cites W2096830865 @default.
• W3082013852 cites W2102603631 @default.
• W3082013852 cites W2108921801 @default.
• W3082013852 cites W2124026197 @default.
• W3082013852 cites W2148209490 @default.
• W3082013852 cites W2168506793 @default.
• W3082013852 cites W2168974130 @default.
• W3082013852 cites W2171981522 @default.
• W3082013852 cites W2180229411 @default.
• W3082013852 cites W2256083943 @default.
• W3082013852 cites W2263707531 @default.
• W3082013852 cites W2324824455 @default.
• W3082013852 cites W2333941380 @default.
• W3082013852 cites W2612477546 @default.
• W3082013852 cites W2752023984 @default.
• W3082013852 cites W2792044715 @default.
• W3082013852 cites W2792346619 @default.
• W3082013852 cites W2795150804 @default.
• W3082013852 cites W2887902800 @default.
• W3082013852 cites W2888260836 @default.
• W3082013852 cites W2889161855 @default.
• W3082013852 cites W2895841615 @default.
• W3082013852 cites W2913652975 @default.
• W3082013852 cites W2943878465 @default.
• W3082013852 cites W2955305973 @default.
• W3082013852 cites W2956726272 @default.
• W3082013852 cites W2986136624 @default.
• W3082013852 cites W2990176834 @default.
• W3082013852 cites W3009004529 @default.
• W3082013852 cites W4255677297 @default.
• W3082013852 doi "https://doi.org/10.1002/prot.25997" @default.
• W3082013852 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32860271" @default.
• W3082013852 hasPublicationYear "2020" @default.
• W3082013852 type Work @default.
• W3082013852 sameAs 3082013852 @default.
• W3082013852 citedByCount "2" @default.
• W3082013852 countsByYear W30820138522022 @default.
• W3082013852 crossrefType "journal-article" @default.
• W3082013852 hasAuthorship W3082013852A5056305582 @default.
• W3082013852 hasAuthorship W3082013852A5058577900 @default.
• W3082013852 hasAuthorship W3082013852A5086461817 @default.
• W3082013852 hasBestOaLocation W30820138522 @default.
• W3082013852 hasConcept C104317684 @default.
• W3082013852 hasConcept C119157956 @default.
• W3082013852 hasConcept C133936738 @default.
• W3082013852 hasConcept C142724271 @default.
• W3082013852 hasConcept C185592680 @default.
• W3082013852 hasConcept C2776111603 @default.
• W3082013852 hasConcept C2777975735 @default.
• W3082013852 hasConcept C2778679867 @default.
• W3082013852 hasConcept C2779649592 @default.
• W3082013852 hasConcept C2780374374 @default.
• W3082013852 hasConcept C2781069245 @default.
• W3082013852 hasConcept C501593827 @default.
• W3082013852 hasConcept C523546767 @default.
• W3082013852 hasConcept C54355233 @default.
• W3082013852 hasConcept C55493867 @default.
• W3082013852 hasConcept C60987743 @default.
• W3082013852 hasConcept C71924100 @default.
• W3082013852 hasConcept C79879829 @default.
• W3082013852 hasConcept C86803240 @default.
• W3082013852 hasConcept C89423630 @default.
• W3082013852 hasConceptScore W3082013852C104317684 @default.
• W3082013852 hasConceptScore W3082013852C119157956 @default.
• W3082013852 hasConceptScore W3082013852C133936738 @default.
• W3082013852 hasConceptScore W3082013852C142724271 @default.
• W3082013852 hasConceptScore W3082013852C185592680 @default.
• W3082013852 hasConceptScore W3082013852C2776111603 @default.
• W3082013852 hasConceptScore W3082013852C2777975735 @default.
• W3082013852 hasConceptScore W3082013852C2778679867 @default.
• W3082013852 hasConceptScore W3082013852C2779649592 @default.
• W3082013852 hasConceptScore W3082013852C2780374374 @default.
• W3082013852 hasConceptScore W3082013852C2781069245 @default.
• W3082013852 hasConceptScore W3082013852C501593827 @default.
• W3082013852 hasConceptScore W3082013852C523546767 @default.
• W3082013852 hasConceptScore W3082013852C54355233 @default.
• W3082013852 hasConceptScore W3082013852C55493867 @default.
• W3082013852 hasConceptScore W3082013852C60987743 @default.
• W3082013852 hasConceptScore W3082013852C71924100 @default.
• W3082013852 hasConceptScore W3082013852C79879829 @default.

• next