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- W3082034106 abstract "Spin trapping with cyclic nitrones coupled to electron paramagnetic resonance (EPR) enables the detection and characterization of oxygen-derived free radicals, such as superoxide and hydroxyl radicals, in living cells. Detection is usually performed on cell suspensions introduced in glass capillaries, gas-permeable tubing, or flat cells, even when cells normally require attachment for growth. However, radical production may be influenced by cell adhesion, while enzymatic or mechanical cell harvesting may damage the cells and alter their metabolic rates. Here, we describe the detection on adherent cells attached to microscope coverslip glasses. This method preserves cell integrity, ensures near physiological conditions for naturally adherent cells, and is relatively simple to set up. Up to 12 conditions can be screened in half a day using a single batch of culture cells." @default.
- W3082034106 created "2020-09-08" @default.
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- W3082034106 creator A5083040282 @default.
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- W3082034106 date "2020-08-29" @default.
- W3082034106 modified "2023-10-18" @default.
- W3082034106 title "Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones" @default.
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- W3082034106 doi "https://doi.org/10.1007/978-1-0716-0896-8_13" @default.
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