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• W3082086760 endingPage "165951" @default.
• W3082086760 startingPage "165951" @default.
• W3082086760 abstract "Trypanosoma cruzi has a complex life cycle involving four life stages: the replicative epimastigotes and metacyclic trypomastigotes in the invertebrate host digestive tract, and intracellular amastigotes and bloodstream trypomastigotes in the mammalian host. Trypomastigotes can invade any nucleated cell, including macrophages, which produce ROS that enhance intracellular infection. However, how ROS modulate T. cruzi infection in the mammalian cell remains unclear. Therefore, the present work aimed to investigate the role of ROS during the stimulation of amastigogenesis in vitro . Our results showed that H 2 O 2 improves the differentiation process in vitro and that it was impaired by Peg-Catalase. However, the antioxidants GSH and NAC had no influence on induced amastigogenesis, which suggests the specificity of H 2 O 2 to increase intracellular differentiation. Amastigogenesis physiologically occurs in low pH, thus we investigated whether parasites are able to produce ROS during amastigogenesis. Interestingly, after 60 min of differentiation induction in vitro , we observed an increase in H 2 O 2 production, which was inhibited by the mitochondrial-targeted antioxidant, mitoTEMPO and Cyclosporine A (a mitochondrial permeability transition pore -mPTP- inhibitor), suggesting mitochondrion as a H 2 O 2 source. Indeed, quantitative real time (qPCR) showed an increase of the mitochondrial superoxide dismutase (FeSODA) gene expression after 60 min of induced amastigogenesis, reinforcing the hypothesis of mitochondrial ROS induction during intracellular differentiation of T. cruzi . The reduction of cellular respiration and the decreased ΔΨm observed during amastigogenesis can explain the increased mitochondrial ROS through mPTP opening. In conclusion, our results suggest that H 2 O 2 is involved in the amastigogenesis of T. cruzi . • Hydrogen peroxide favors amastigogenesis of Trypanosoma cruzi in vitro . • The mitochondrion is dysfunctional during amastigogenesis in vitro . • FeSODA gene expression is regulated positively along of differentiation of T. cruzi ." @default.
• W3082086760 created "2020-09-08" @default.
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• W3082086760 date "2020-12-01" @default.
• W3082086760 modified "2023-09-26" @default.
• W3082086760 title "New perspectives for hydrogen peroxide in the amastigogenesis of Trypanosoma cruzi in vitro" @default.
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• W3082086760 doi "https://doi.org/10.1016/j.bbadis.2020.165951" @default.
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