FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3082119675> ?p ?o ?g. }

• W3082119675 endingPage "518" @default.
• W3082119675 startingPage "518" @default.
• W3082119675 abstract "HomePlant DiseaseVol. 105, No. 2The Occurrence of Pea Enation Mosaic Virus 1 and Pea Enation Mosaic Virus 2 from Disease-Affected Pea Fields in China PreviousNext DISEASE NOTES OPENOpen Access licenseThe Occurrence of Pea Enation Mosaic Virus 1 and Pea Enation Mosaic Virus 2 from Disease-Affected Pea Fields in ChinaXiaojiao Chen, Kehua Li, Henming Luo, Shu Han, Qinhai Liu, Guanlin Tan, Ruhui Li, and Fan LiXiaojiao ChenState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaSearch for more papers by this author, Kehua LiState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaSearch for more papers by this author, Henming LuoState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaSearch for more papers by this author, Shu HanCollege of Landscape Architecture and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaSearch for more papers by this author, Qinhai LiuState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaSearch for more papers by this author, Guanlin TanModern Education Technology Center, Yunnan Agricultural University, Kunming 650201, ChinaSearch for more papers by this author, Ruhui Lihttp://orcid.org/0000-0002-9271-4208USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD 20705, U.S.A.Search for more papers by this author, and Fan Li†Corresponding author: F. Li; E-mail Address: fanlikm@126.comhttp://orcid.org/0000-0003-3084-5912State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaSearch for more papers by this author AffiliationsAuthors and Affiliations Xiaojiao Chen1 Kehua Li1 Henming Luo1 Shu Han2 Qinhai Liu1 Guanlin Tan3 Ruhui Li4 Fan Li1 † 1State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China 2College of Landscape Architecture and Horticulture, Yunnan Agricultural University, Kunming 650201, China 3Modern Education Technology Center, Yunnan Agricultural University, Kunming 650201, China 4USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD 20705, U.S.A. Published Online:8 Dec 2020https://doi.org/10.1094/PDIS-05-20-1123-PDNAboutSectionsView articlePDFPDF Plus ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articlePea (Pisum sativum L.) is an economically important legume crop that is commonly used as dried beans, fresh peas, pods, and shoots (Guo et al. 2009). Pea enation mosaic is an important virus disease of pea caused by two viruses in an obligate symbiosis, Pea enation mosaic virus 1 (Enamovirus, Luteoviridae) and Pea enation mosaic virus 2 (Umbravirus, Tombusviridae) (Hema et al. 2014). In November 2019, foliar yellow mosaic and vein enation symptoms were observed from pea plants in five fields of Honghe autonomous prefecture, Yunnan province, China. Incidence of symptomatic plants ranged from 20 to 40% and was distributed in both small and large fields. Leaves with typical virus-like symptoms were collected from five symptomatic pea plants in two fields and used for total RNA extraction. The five extracts of equimolar quantities were pooled into a sample and subjected to high-throughput sequencing (HTS) by the Illumina HiSeq system. Analyses of raw RNA reads were performed using CLC Genomics Workbench 12 (Qiagen). A total of 60,009,746 RNA reads were obtained from the sample, and de novo assembly of the reads using the CLC Genomics generated 88,105 contigs. BLASTN searches revealed the presence of contigs with high similarities to pea enation mosaic virus 1 (PEMV1), pea enation mosaic virus 2 (PEMV2), pea seed-borne mosaic virus, and bean yellow mosaic virus. To confirm the presence of PEMV-1 and PEMV-2 in the samples, two virus-specific primer pairs were designed based on the contig sequences obtained by HTS in this study. Primer pairs PEMV-1F/PEMV-1R (5′-ATGCCGACTAGATCGAAATC-3′/5′-TCAGAGGGAGGCATTCATTA-3′) that flank the cp gene of PEMV-1 and PEMV-2F/PEMV-2R (5′-ATGACGATAATCATTAATG-3′/5′-TCACCCGTAGTGAGAGGCA-3′) that target the ORF3 region of PEMV-2 were used to amplify the two viruses by reverse transcription polymerase chain reaction (RT-PCR). DNA fragments of the expected sizes (PEMV-1, 570 bp; PEMV-2, 693 bp) were amplified from all five samples. The RT-PCR products were cloned and sequenced. Sequence analysis showed that the 570-bp amplicon (MT481989) shared the highest nucleotide sequence identity of 98.95% with PEMV-1 (Z48507), whereas the 693-bp fragment (MT481990) had the highest nucleotide sequence identity of 97.4% with PEMV-2 isolate JKI (MK948534). One gram of the symptomatic leaves from each of the five plants was homogenized with 5 ml of 0.01 M phosphate-buffered saline (PBS buffer), pH 7.0. Each of the resulting saps was used to inoculate onto five healthy pea seedlings. A total of 25 healthy pea seedlings were inoculated, and 16 inoculated plants developed yellowing and mottling at 10 days postinoculation (dpi); no symptoms were observed on control plants inoculated only with PBS buffer. The formation of typical enation was observed along the veins of the lower side of the symptomatic leaves of the inoculated plants at 30 dpi. PEMV-1 and PEMV-2 infection were confirmed by RT-PCR assays using the specific primer pairs described above. Although the presence of the pea enation mosaic virus complex was suspected in China based on symptomatology (Brunt et al. 1997), to our knowledge this is the first molecular and biological confirmation of PEMV-1 and PEMV-2 occurrence in China. The coinfection of PEMV-1 and PEMV-2 usually causes severe yield losses; therefore, integration of detection and control measures is important in pea production regions where the two viruses occurred.The author(s) declare no conflict of interest.References:Brunt, A. A., et al., eds. 1997. Plant Viruses Online: Description and Lists from the VIDE Database. CAB International, Cambridge, U.K. Google ScholarGuo, P. Y., et al. 2009. Anhui Agric. Sci. Bull. 158:87 (in Chinese). Google ScholarHema, M., et al. 2014. Adv. Virus Res. 90:431. https://doi.org/10.1016/B978-0-12-801246-8.00009-3 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: This research was supported by the Natural Science Foundation of Yunnan Province, China (2018FD024) and the Program for Innovative Research Team (in Science and Technology) in University of Yunnan Province (Yunjiaoke 2014-22).DetailsFiguresLiterature CitedRelated Vol. 105, No. 2 February 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionDeformation of a bud in blackcurrant after infestation with the mite Cecidophyopsis ribis, Acari: Eriophyidae (Špak et al.). Photo credit: J. Špak. Symptoms of sugar beet rubbery taproot disease (Ćurčić et al.). Photo credit: B. Duduk. Metrics Downloaded 768 times Article History Issue Date: 24 Feb 2021Published: 8 Dec 2020First Look: 27 Aug 2020Accepted: 25 Aug 2020 Page: 518 Information© 2021 The American Phytopathological SocietyFundingNatural Science Foundation of Yunnan Province, ChinaGrant/Award Number: 2018FD024Program for Innovative Research Team (in Science and Technology) in University of Yunnan ProvinceGrant/Award Number: Yunjiaoke 2014-22Keywordsviruses and viroidsPEMV-1 and PEMV-2oilseeds and legumespathogen detectionThe author(s) declare no conflict of interest.PDF download" @default.
• W3082119675 created "2020-09-08" @default.
• W3082119675 creator A5010236216 @default.
• W3082119675 creator A5028238474 @default.
• W3082119675 creator A5029089099 @default.
• W3082119675 creator A5047675558 @default.
• W3082119675 creator A5050038941 @default.
• W3082119675 creator A5058247310 @default.
• W3082119675 creator A5080037560 @default.
• W3082119675 creator A5081842390 @default.
• W3082119675 date "2021-02-01" @default.
• W3082119675 modified "2023-10-10" @default.
• W3082119675 title "The Occurrence of Pea Enation Mosaic Virus 1 and Pea Enation Mosaic Virus 2 from Disease-Affected Pea Fields in China" @default.
• W3082119675 cites W184700228 @default.
• W3082119675 doi "https://doi.org/10.1094/pdis-05-20-1123-pdn" @default.
• W3082119675 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32854603" @default.
• W3082119675 hasPublicationYear "2021" @default.
• W3082119675 type Work @default.
• W3082119675 sameAs 3082119675 @default.
• W3082119675 citedByCount "3" @default.
• W3082119675 countsByYear W30821196752022 @default.
• W3082119675 countsByYear W30821196752023 @default.
• W3082119675 crossrefType "journal-article" @default.
• W3082119675 hasAuthorship W3082119675A5010236216 @default.
• W3082119675 hasAuthorship W3082119675A5028238474 @default.
• W3082119675 hasAuthorship W3082119675A5029089099 @default.
• W3082119675 hasAuthorship W3082119675A5047675558 @default.
• W3082119675 hasAuthorship W3082119675A5050038941 @default.
• W3082119675 hasAuthorship W3082119675A5058247310 @default.
• W3082119675 hasAuthorship W3082119675A5080037560 @default.
• W3082119675 hasAuthorship W3082119675A5081842390 @default.
• W3082119675 hasBestOaLocation W30821196751 @default.
• W3082119675 hasConcept C109110057 @default.
• W3082119675 hasConcept C159047783 @default.
• W3082119675 hasConcept C2522874641 @default.
• W3082119675 hasConcept C2780951447 @default.
• W3082119675 hasConcept C59822182 @default.
• W3082119675 hasConcept C86803240 @default.
• W3082119675 hasConceptScore W3082119675C109110057 @default.
• W3082119675 hasConceptScore W3082119675C159047783 @default.
• W3082119675 hasConceptScore W3082119675C2522874641 @default.
• W3082119675 hasConceptScore W3082119675C2780951447 @default.
• W3082119675 hasConceptScore W3082119675C59822182 @default.
• W3082119675 hasConceptScore W3082119675C86803240 @default.
• W3082119675 hasIssue "2" @default.
• W3082119675 hasLocation W30821196751 @default.
• W3082119675 hasLocation W30821196752 @default.
• W3082119675 hasOpenAccess W3082119675 @default.
• W3082119675 hasPrimaryLocation W30821196751 @default.
• W3082119675 hasRelatedWork W1874513932 @default.
• W3082119675 hasRelatedWork W2016551366 @default.
• W3082119675 hasRelatedWork W2094230406 @default.
• W3082119675 hasRelatedWork W2111970140 @default.
• W3082119675 hasRelatedWork W2123163123 @default.
• W3082119675 hasRelatedWork W2138564085 @default.
• W3082119675 hasRelatedWork W2147145791 @default.
• W3082119675 hasRelatedWork W2188759708 @default.
• W3082119675 hasRelatedWork W2314757260 @default.
• W3082119675 hasRelatedWork W4386371748 @default.
• W3082119675 hasVolume "105" @default.
• W3082119675 isParatext "false" @default.
• W3082119675 isRetracted "false" @default.
• W3082119675 magId "3082119675" @default.
• W3082119675 workType "article" @default.