Matches in SemOpenAlex for { <https://semopenalex.org/work/W3082204401> ?p ?o ?g. }
- W3082204401 abstract "Abstract Myxococcus xanthus displays two types of motilities i.e. Social (S) and Adventurous (A). The pole-to-pole reversals of these motility regulator proteins is the key to this process. Here, we determined ~1.85 Å resolution crystal structure of MglC, which revealed that despite sharing <9% sequence identity, both MglB and MglC adopt R egulatory L ight C hain 7 (RLC7) family fold. Interestingly, MglC is structurally unique compared to the other known RLC7 family proteins having ~30°-40° shift in the orientation of functionally important α2 helix. Using isothermal titration calorimetry and gel filtration chromatography, we show that MglC binds MglB in 2:4 stoichiometry with submicromolar range dissociation constant. Using combination of small angle X-ray scattering and molecular docking studies, we show that MglBC complex is formed by MglC homodimer sandwiched between two homodimers of MglB. In Brief Kapoor et al . report the crystal structure of Myxococcus xanthus MglC, a R oadblock L ight C hain 7 (RLC7) family protein, involved in polarity reversal. The structure reveals a distinct orientation of α2 helix compared to other RLC7 proteins. They also demonstrate that MglC binds a GTPase activating protein, MglB, with submicromolar range dissociation constant. Highlights MglC adopts RLC7 fold and has distinct structural features. MglC interacts MglB to form a stable complex having submicromolar range dissociation constant. MglC homodimer is sandwiched between two MglB homodimers to form a 2:4 stoichiometric complex." @default.
- W3082204401 created "2020-09-08" @default.
- W3082204401 creator A5032228746 @default.
- W3082204401 creator A5069479089 @default.
- W3082204401 creator A5076435991 @default.
- W3082204401 creator A5081288778 @default.
- W3082204401 creator A5085350939 @default.
- W3082204401 date "2020-08-28" @default.
- W3082204401 modified "2023-09-23" @default.
- W3082204401 title "Structural characterization of Myxococcus xanthus MglC, a component of polarity control system, and its interactions with MglB" @default.
- W3082204401 cites W1578471917 @default.
- W3082204401 cites W1816674519 @default.
- W3082204401 cites W1899503782 @default.
- W3082204401 cites W1961599893 @default.
- W3082204401 cites W1964568191 @default.
- W3082204401 cites W1966797058 @default.
- W3082204401 cites W1973035681 @default.
- W3082204401 cites W1975009150 @default.
- W3082204401 cites W1988283355 @default.
- W3082204401 cites W1991357892 @default.
- W3082204401 cites W1992685818 @default.
- W3082204401 cites W2009386800 @default.
- W3082204401 cites W2012332337 @default.
- W3082204401 cites W2029582401 @default.
- W3082204401 cites W2035503835 @default.
- W3082204401 cites W2038840577 @default.
- W3082204401 cites W2051141334 @default.
- W3082204401 cites W2053615988 @default.
- W3082204401 cites W2054099099 @default.
- W3082204401 cites W2054668663 @default.
- W3082204401 cites W2055874155 @default.
- W3082204401 cites W2060513085 @default.
- W3082204401 cites W2065088745 @default.
- W3082204401 cites W2065326095 @default.
- W3082204401 cites W2065388862 @default.
- W3082204401 cites W2069663555 @default.
- W3082204401 cites W2070038884 @default.
- W3082204401 cites W2090364460 @default.
- W3082204401 cites W2092636253 @default.
- W3082204401 cites W2108921801 @default.
- W3082204401 cites W2109834023 @default.
- W3082204401 cites W2121956971 @default.
- W3082204401 cites W2134460102 @default.
- W3082204401 cites W2144081223 @default.
- W3082204401 cites W2144998676 @default.
- W3082204401 cites W2147949826 @default.
- W3082204401 cites W2149885731 @default.
- W3082204401 cites W2150444353 @default.
- W3082204401 cites W2159211495 @default.
- W3082204401 cites W2160570188 @default.
- W3082204401 cites W2162800127 @default.
- W3082204401 cites W2168621448 @default.
- W3082204401 cites W2168974130 @default.
- W3082204401 cites W2191906954 @default.
- W3082204401 cites W2204502255 @default.
- W3082204401 cites W2320033710 @default.
- W3082204401 cites W2376573086 @default.
- W3082204401 cites W2480100570 @default.
- W3082204401 cites W2561826764 @default.
- W3082204401 cites W2572080171 @default.
- W3082204401 cites W2597519458 @default.
- W3082204401 cites W2707646777 @default.
- W3082204401 cites W2732000326 @default.
- W3082204401 cites W2767173427 @default.
- W3082204401 cites W2792184967 @default.
- W3082204401 cites W2805621788 @default.
- W3082204401 cites W2884645260 @default.
- W3082204401 cites W2945894003 @default.
- W3082204401 cites W2973457155 @default.
- W3082204401 cites W2975691003 @default.
- W3082204401 cites W2980986045 @default.
- W3082204401 cites W2990514693 @default.
- W3082204401 cites W3048286498 @default.
- W3082204401 cites W4236236547 @default.
- W3082204401 doi "https://doi.org/10.1101/2020.08.27.270058" @default.
- W3082204401 hasPublicationYear "2020" @default.
- W3082204401 type Work @default.
- W3082204401 sameAs 3082204401 @default.
- W3082204401 citedByCount "0" @default.
- W3082204401 crossrefType "posted-content" @default.
- W3082204401 hasAuthorship W3082204401A5032228746 @default.
- W3082204401 hasAuthorship W3082204401A5069479089 @default.
- W3082204401 hasAuthorship W3082204401A5076435991 @default.
- W3082204401 hasAuthorship W3082204401A5081288778 @default.
- W3082204401 hasAuthorship W3082204401A5085350939 @default.
- W3082204401 hasBestOaLocation W30822044011 @default.
- W3082204401 hasConcept C104317684 @default.
- W3082204401 hasConcept C12554922 @default.
- W3082204401 hasConcept C143065580 @default.
- W3082204401 hasConcept C156911925 @default.
- W3082204401 hasConcept C170493617 @default.
- W3082204401 hasConcept C185592680 @default.
- W3082204401 hasConcept C2777120639 @default.
- W3082204401 hasConcept C55493867 @default.
- W3082204401 hasConcept C71240020 @default.
- W3082204401 hasConcept C74998103 @default.
- W3082204401 hasConcept C8010536 @default.
- W3082204401 hasConcept C86803240 @default.
- W3082204401 hasConceptScore W3082204401C104317684 @default.
- W3082204401 hasConceptScore W3082204401C12554922 @default.