Matches in SemOpenAlex for { <https://semopenalex.org/work/W3082205315> ?p ?o ?g. }
- W3082205315 endingPage "10273" @default.
- W3082205315 startingPage "10262" @default.
- W3082205315 abstract "Tumor-derived extracellular vesicle (TEV) protein biomarkers facilitate cancer diagnosis and prognostic evaluations. However, the lack of reliable and convenient quantitative methods for evaluating TEV proteins prevents their clinical application. Methods: Here, based on dual amplification of hybridization chain reaction (HCR) and CRISPR-Cas12a, we developed the apta-HCR-CRISPR assay for direct high-sensitivity detection of TEV proteins. The TEV protein-targeted aptamer was amplified by HCR to produce a long-repeated sequence comprising multiple CRISPR RNA (crRNA) targetable barcodes, and the signals were further amplified by CRISPR-Cas12a collateral cleavage activities, resulting in a fluorescence signal. Results: The established strategy was verified by detecting the TEV protein markers nucleolin and programmed death ligand 1 (PD-L1). Both achieved limit of detection (LOD) values as low as 102 particles/µL, which is at least 104-fold more sensitive than aptamer-ELISA and 102-fold more sensitive than apta-HCR-ELISA. We directly applied our assay to a clinical analysis of circulating TEVs from 50 µL of serum, revealing potential applications of nucleolin+ TEVs for nasopharyngeal carcinoma cancer (NPC) diagnosis and PD-L1+ TEVs for therapeutic monitoring. Conclusion: The platform was simple and easy to operate, and this approach should be useful for the highly sensitive and versatile quantification of TEV proteins in clinical samples." @default.
- W3082205315 created "2020-09-08" @default.
- W3082205315 creator A5001768275 @default.
- W3082205315 creator A5007442731 @default.
- W3082205315 creator A5016295149 @default.
- W3082205315 creator A5028424745 @default.
- W3082205315 creator A5030766995 @default.
- W3082205315 creator A5046430731 @default.
- W3082205315 creator A5061999013 @default.
- W3082205315 creator A5079594267 @default.
- W3082205315 creator A5085856711 @default.
- W3082205315 date "2020-01-01" @default.
- W3082205315 modified "2023-10-17" @default.
- W3082205315 title "An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification" @default.
- W3082205315 cites W1762620449 @default.
- W3082205315 cites W1917885059 @default.
- W3082205315 cites W1997686479 @default.
- W3082205315 cites W2000455734 @default.
- W3082205315 cites W2010082025 @default.
- W3082205315 cites W2027195751 @default.
- W3082205315 cites W2043915025 @default.
- W3082205315 cites W2071829817 @default.
- W3082205315 cites W2098259366 @default.
- W3082205315 cites W2148304424 @default.
- W3082205315 cites W2154093209 @default.
- W3082205315 cites W2167497075 @default.
- W3082205315 cites W2186378789 @default.
- W3082205315 cites W2259481044 @default.
- W3082205315 cites W2260715625 @default.
- W3082205315 cites W2283764701 @default.
- W3082205315 cites W2327538814 @default.
- W3082205315 cites W2330004628 @default.
- W3082205315 cites W2512899508 @default.
- W3082205315 cites W2565550971 @default.
- W3082205315 cites W2581984518 @default.
- W3082205315 cites W2592740738 @default.
- W3082205315 cites W2620625960 @default.
- W3082205315 cites W2767538197 @default.
- W3082205315 cites W2769948007 @default.
- W3082205315 cites W2785904295 @default.
- W3082205315 cites W2787024379 @default.
- W3082205315 cites W2793970395 @default.
- W3082205315 cites W2794357293 @default.
- W3082205315 cites W2801783434 @default.
- W3082205315 cites W2887249435 @default.
- W3082205315 cites W2890807645 @default.
- W3082205315 cites W2894273932 @default.
- W3082205315 cites W2897734489 @default.
- W3082205315 cites W2902714386 @default.
- W3082205315 cites W2904267251 @default.
- W3082205315 cites W2904802348 @default.
- W3082205315 cites W2909018635 @default.
- W3082205315 cites W2909923156 @default.
- W3082205315 cites W2940355051 @default.
- W3082205315 cites W2950877338 @default.
- W3082205315 cites W2951300836 @default.
- W3082205315 cites W2961148088 @default.
- W3082205315 cites W2963049400 @default.
- W3082205315 cites W2964187307 @default.
- W3082205315 cites W2965026412 @default.
- W3082205315 cites W2967373993 @default.
- W3082205315 cites W2970391528 @default.
- W3082205315 cites W2971843398 @default.
- W3082205315 cites W2972626463 @default.
- W3082205315 cites W2978292797 @default.
- W3082205315 cites W2981217376 @default.
- W3082205315 cites W2992478910 @default.
- W3082205315 cites W2995017830 @default.
- W3082205315 cites W2998905102 @default.
- W3082205315 cites W3003804672 @default.
- W3082205315 cites W3006528425 @default.
- W3082205315 cites W3006553874 @default.
- W3082205315 cites W3011255507 @default.
- W3082205315 cites W3015659943 @default.
- W3082205315 cites W3016213906 @default.
- W3082205315 cites W3016964118 @default.
- W3082205315 cites W3021211921 @default.
- W3082205315 cites W3028142906 @default.
- W3082205315 doi "https://doi.org/10.7150/thno.49047" @default.
- W3082205315 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7481432" @default.
- W3082205315 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32929347" @default.
- W3082205315 hasPublicationYear "2020" @default.
- W3082205315 type Work @default.
- W3082205315 sameAs 3082205315 @default.
- W3082205315 citedByCount "71" @default.
- W3082205315 countsByYear W30822053152021 @default.
- W3082205315 countsByYear W30822053152022 @default.
- W3082205315 countsByYear W30822053152023 @default.
- W3082205315 crossrefType "journal-article" @default.
- W3082205315 hasAuthorship W3082205315A5001768275 @default.
- W3082205315 hasAuthorship W3082205315A5007442731 @default.
- W3082205315 hasAuthorship W3082205315A5016295149 @default.
- W3082205315 hasAuthorship W3082205315A5028424745 @default.
- W3082205315 hasAuthorship W3082205315A5030766995 @default.
- W3082205315 hasAuthorship W3082205315A5046430731 @default.
- W3082205315 hasAuthorship W3082205315A5061999013 @default.
- W3082205315 hasAuthorship W3082205315A5079594267 @default.
- W3082205315 hasAuthorship W3082205315A5085856711 @default.