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- W3082816853 abstract "Long noncoding RNAs (lncRNAs) are a recently discovered class of RNA that have diverse intracellular regulatory and structural roles. Because of their wide assortment of functions, lncRNAs can have varied distributions in the nucleus and/or cytoplasm of a cell. However, even though tens of thousands of human lncRNAs have been identified, currently less than 3% have empirically validated functions. RNA knockdown is now a relatively commonplace laboratory technique used to functionally characterize an RNA. These techniques (most commonly antisense therapy and RNA interference) can even have therapeutic benefit to treat a wide variety of genetic or infectious diseases as evidenced by the several RNA knockdown reagents currently in clinical trials. This protocol describes the use of validated gapmer antisense oligonucleotides (ASOs) to knockdown human MALAT1, a nuclear-retained lncRNA that is upregulated in multiple cancer cells. Methods used include cationic lipid transfection into HeLa cells, RNA isolation, and RT-qPCR analysis of the RNA knockdown levels." @default.
- W3082816853 created "2020-09-08" @default.
- W3082816853 creator A5029954209 @default.
- W3082816853 creator A5058754373 @default.
- W3082816853 date "2020-01-01" @default.
- W3082816853 modified "2023-09-23" @default.
- W3082816853 title "Tips for Successful lncRNA Knockdown Using Gapmers" @default.
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- W3082816853 doi "https://doi.org/10.1007/978-1-0716-0771-8_9" @default.
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