FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3083100836> ?p ?o ?g. }

• W3083100836 endingPage "278" @default.
• W3083100836 startingPage "278" @default.
• W3083100836 abstract "Abstract One of the known circular RNAs (circRNAs) functions is to sponge microRNAs (miRNAs). Studies reported dysregulated mRNA-circRNA-miRNAs axes as biomarker signatures in breast cancer (BC). Signatures in early-onset BC have not been characterized, while its incidence increased drastically worldwide. To address this gap, we performed circRNAs microarrays and miRNA-Sequencing on 3D breast epithelial risk-progression HMT-3522 S1 (S1) culture model to identify dysregulated post-transcriptional axes. S1 (non-neoplastic) cells form fully-polarized epithelium and closely mimic normal in vivo mammary epithelial morphology, while (pre-tumorigenic) Cx43KO-S1 (S1 silenced with shRNA for gap junction Cx43) lose epithelial polarity, multi-layer and mimic tumor-initiated in vivo mammary epithelial morphology. We detected through circRNA microarray (Arraystar Human circRNA Array V2) 75 significantly upregulated and 46 significantly downregulated circRNAs (Fold Change &gt; 2, p-value &lt; 0.05) in 6 samples of Cx43KO-S1 versus S1 cells in 3D. We then selected 10 upregulated and 8 downregulated circRNAs for validation. Of these, hsa_circ_0007961 and hsa_circ_0081481 were most significantly upregulated while hsa_circ_0060055, hsa_circ_0005185, hsa_circ_0083442 and hsa_circ_0077755 were most significantly downregulated by RT-qPCR. We focus on “possible” sponging activity of validated circRNAs to their predicted (in silico) target miRNAs, and cross-compared their dysregulation to our validated miRNA datasets (from young patients and 3D cell line). When circRNAs are upregulated, they downregulate the function of their sponged miRNAs, hence preventing them from deregulating their target mRNAs. For upregulated hsa_circ_0007961 (originating from SPRED2 mRNA), its predicted miR-99a-3p was indeed downregulated in young Lebanese and matched U.S. BC patient tissues (and in Cx43KO-S1 versus S1 microarrays) and predicted miR-653 possessed treatment potential in BC cells. For the second upregulated hsa_circ_0081481 (originating from FBXO24 mRNA), its predicted miR-3960 was exclusively dysregulated in young Lebanese BC patients. For downregulated hsa_circ_0077755 (originating from Cx43mRNA), its predicted miR-182 was commonly upregulated in young Lebanese and matched US patients and predicted miR-203a-3p was reported as promising prognostic biomarker in triple negative BC. Additionally, all predicted miRNAs targeted by all detected circRNAs were predominantly enriched in pathways in cancer. Thus, we suggest that our 3D risk-progression culture model might help delineate post-transcriptional axes to better understand early-onset BC initiation. We reveal three validated axes: i) SPRED2/hsa_circ_0007961/miR-99a-3p or miR-653-5p, ii) FBXO24/hsa_circ_0081481/miR-3960 and iii) Cx43/hsa_circ_0077755/miR-182 or miR-203, which could serve as potential biomarker signatures in early-onset BC. Citation Format: Nataly Naser Al Deen, Nadia Atallah Lanman, Shirisha Chittiboyina, Sophie Lelièvre, Heinrich Zu Dohna, Mounir AbouHaidar, Rabih Talhouk. Deciphering circular RNA axes in early-onset breast cancer using a breast epithelial risk-progression 3D culture model [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 278." @default.
• W3083100836 created "2020-09-11" @default.
• W3083100836 creator A5007256019 @default.
• W3083100836 creator A5024391740 @default.
• W3083100836 creator A5049467929 @default.
• W3083100836 creator A5053110684 @default.
• W3083100836 creator A5054488410 @default.
• W3083100836 creator A5055444545 @default.
• W3083100836 creator A5090412711 @default.
• W3083100836 date "2020-08-15" @default.
• W3083100836 modified "2023-10-16" @default.
• W3083100836 title "Abstract 278: Deciphering circular RNA axes in early-onset breast cancer using a breast epithelial risk-progression 3D culture model" @default.
• W3083100836 doi "https://doi.org/10.1158/1538-7445.am2020-278" @default.
• W3083100836 hasPublicationYear "2020" @default.
• W3083100836 type Work @default.
• W3083100836 sameAs 3083100836 @default.
• W3083100836 citedByCount "1" @default.
• W3083100836 countsByYear W30831008362022 @default.
• W3083100836 crossrefType "journal-article" @default.
• W3083100836 hasAuthorship W3083100836A5007256019 @default.
• W3083100836 hasAuthorship W3083100836A5024391740 @default.
• W3083100836 hasAuthorship W3083100836A5049467929 @default.
• W3083100836 hasAuthorship W3083100836A5053110684 @default.
• W3083100836 hasAuthorship W3083100836A5054488410 @default.
• W3083100836 hasAuthorship W3083100836A5055444545 @default.
• W3083100836 hasAuthorship W3083100836A5090412711 @default.
• W3083100836 hasConcept C104317684 @default.
• W3083100836 hasConcept C121608353 @default.
• W3083100836 hasConcept C127561419 @default.
• W3083100836 hasConcept C145059251 @default.
• W3083100836 hasConcept C150194340 @default.
• W3083100836 hasConcept C153911025 @default.
• W3083100836 hasConcept C186836561 @default.
• W3083100836 hasConcept C207001950 @default.
• W3083100836 hasConcept C2775893923 @default.
• W3083100836 hasConcept C2775905019 @default.
• W3083100836 hasConcept C502942594 @default.
• W3083100836 hasConcept C530470458 @default.
• W3083100836 hasConcept C54355233 @default.
• W3083100836 hasConcept C8415881 @default.
• W3083100836 hasConcept C86803240 @default.
• W3083100836 hasConcept C95444343 @default.
• W3083100836 hasConceptScore W3083100836C104317684 @default.
• W3083100836 hasConceptScore W3083100836C121608353 @default.
• W3083100836 hasConceptScore W3083100836C127561419 @default.
• W3083100836 hasConceptScore W3083100836C145059251 @default.
• W3083100836 hasConceptScore W3083100836C150194340 @default.
• W3083100836 hasConceptScore W3083100836C153911025 @default.
• W3083100836 hasConceptScore W3083100836C186836561 @default.
• W3083100836 hasConceptScore W3083100836C207001950 @default.
• W3083100836 hasConceptScore W3083100836C2775893923 @default.
• W3083100836 hasConceptScore W3083100836C2775905019 @default.
• W3083100836 hasConceptScore W3083100836C502942594 @default.
• W3083100836 hasConceptScore W3083100836C530470458 @default.
• W3083100836 hasConceptScore W3083100836C54355233 @default.
• W3083100836 hasConceptScore W3083100836C8415881 @default.
• W3083100836 hasConceptScore W3083100836C86803240 @default.
• W3083100836 hasConceptScore W3083100836C95444343 @default.
• W3083100836 hasIssue "16_Supplement" @default.
• W3083100836 hasLocation W30831008361 @default.
• W3083100836 hasOpenAccess W3083100836 @default.
• W3083100836 hasPrimaryLocation W30831008361 @default.
• W3083100836 hasRelatedWork W2066009447 @default.
• W3083100836 hasRelatedWork W2132025459 @default.
• W3083100836 hasRelatedWork W2280814768 @default.
• W3083100836 hasRelatedWork W2747105326 @default.
• W3083100836 hasRelatedWork W2904981640 @default.
• W3083100836 hasRelatedWork W3016705944 @default.
• W3083100836 hasRelatedWork W3083100836 @default.
• W3083100836 hasRelatedWork W3096761788 @default.
• W3083100836 hasRelatedWork W3164806319 @default.
• W3083100836 hasRelatedWork W4306409086 @default.
• W3083100836 hasVolume "80" @default.
• W3083100836 isParatext "false" @default.
• W3083100836 isRetracted "false" @default.
• W3083100836 magId "3083100836" @default.
• W3083100836 workType "article" @default.