Matches in SemOpenAlex for { <https://semopenalex.org/work/W3083147072> ?p ?o ?g. }
- W3083147072 endingPage "965" @default.
- W3083147072 startingPage "947" @default.
- W3083147072 abstract "Bacterial flagella have many established roles beyond swimming motility. Despite clear evidence of flagella-dependent adherence, the specificity of the ligands and mechanisms of binding are still debated. In this study, the molecular basis of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium flagella binding to epithelial cell cultures was investigated. Flagella interactions with host cell surfaces were intimate and crossed cellular boundaries as demarcated by actin and membrane labelling. Scanning electron microscopy revealed flagella disappearing into cellular surfaces and transmission electron microscopy of S . Typhiumurium indicated host membrane deformation and disruption in proximity to flagella. Motor mutants of E. coli O157:H7 and S . Typhimurium caused reduced haemolysis compared to wild-type, indicating that membrane disruption was in part due to flagella rotation. Flagella from E. coli O157 (H7), EPEC O127 (H6) and S . Typhimurium (P1 and P2 flagella) were shown to bind to purified intracellular components of the actin cytoskeleton and directly increase in vitro actin polymerization rates. We propose that flagella interactions with host cell membranes and cytoskeletal components may help prime intimate attachment and invasion for E. coli O157:H7 and S . Typhimurium, respectively." @default.
- W3083147072 created "2020-09-11" @default.
- W3083147072 creator A5000572566 @default.
- W3083147072 creator A5005770424 @default.
- W3083147072 creator A5006031956 @default.
- W3083147072 creator A5014850653 @default.
- W3083147072 creator A5024374147 @default.
- W3083147072 creator A5045988291 @default.
- W3083147072 creator A5047383852 @default.
- W3083147072 creator A5054695324 @default.
- W3083147072 creator A5060694215 @default.
- W3083147072 creator A5068325710 @default.
- W3083147072 creator A5071404786 @default.
- W3083147072 creator A5073492235 @default.
- W3083147072 creator A5082254037 @default.
- W3083147072 creator A5082389792 @default.
- W3083147072 creator A5083887972 @default.
- W3083147072 creator A5087534292 @default.
- W3083147072 date "2020-10-01" @default.
- W3083147072 modified "2023-09-25" @default.
- W3083147072 title "The interaction of Escherichia coli O157 :H7 and Salmonella Typhimurium flagella with host cell membranes and cytoskeletal components" @default.
- W3083147072 cites W1519633296 @default.
- W3083147072 cites W1523570743 @default.
- W3083147072 cites W1606913541 @default.
- W3083147072 cites W1655404396 @default.
- W3083147072 cites W1972449088 @default.
- W3083147072 cites W1976100793 @default.
- W3083147072 cites W1978038456 @default.
- W3083147072 cites W1978492395 @default.
- W3083147072 cites W1979513602 @default.
- W3083147072 cites W1985517720 @default.
- W3083147072 cites W1986045394 @default.
- W3083147072 cites W1987691266 @default.
- W3083147072 cites W1988127444 @default.
- W3083147072 cites W1989709167 @default.
- W3083147072 cites W2004888237 @default.
- W3083147072 cites W2005846870 @default.
- W3083147072 cites W2006635484 @default.
- W3083147072 cites W2006990207 @default.
- W3083147072 cites W2011067750 @default.
- W3083147072 cites W2014848186 @default.
- W3083147072 cites W2016635228 @default.
- W3083147072 cites W2026811557 @default.
- W3083147072 cites W2026884617 @default.
- W3083147072 cites W2032155429 @default.
- W3083147072 cites W2036315153 @default.
- W3083147072 cites W2038916450 @default.
- W3083147072 cites W2041410195 @default.
- W3083147072 cites W2046015569 @default.
- W3083147072 cites W2052874989 @default.
- W3083147072 cites W2054919436 @default.
- W3083147072 cites W2058128063 @default.
- W3083147072 cites W2060001563 @default.
- W3083147072 cites W2060196294 @default.
- W3083147072 cites W2071174017 @default.
- W3083147072 cites W2073200952 @default.
- W3083147072 cites W2075948284 @default.
- W3083147072 cites W2096322420 @default.
- W3083147072 cites W2099648551 @default.
- W3083147072 cites W2100782488 @default.
- W3083147072 cites W2101058428 @default.
- W3083147072 cites W2108209554 @default.
- W3083147072 cites W2112659088 @default.
- W3083147072 cites W2113307778 @default.
- W3083147072 cites W2114622395 @default.
- W3083147072 cites W2117080080 @default.
- W3083147072 cites W2117411464 @default.
- W3083147072 cites W2125153220 @default.
- W3083147072 cites W2134153749 @default.
- W3083147072 cites W2134225499 @default.
- W3083147072 cites W2136590188 @default.
- W3083147072 cites W2137055458 @default.
- W3083147072 cites W2140611106 @default.
- W3083147072 cites W2141063937 @default.
- W3083147072 cites W2144994210 @default.
- W3083147072 cites W2150228730 @default.
- W3083147072 cites W2154873371 @default.
- W3083147072 cites W2157747648 @default.
- W3083147072 cites W2164490096 @default.
- W3083147072 cites W2167300120 @default.
- W3083147072 cites W2171228833 @default.
- W3083147072 cites W2179902466 @default.
- W3083147072 cites W2406032681 @default.
- W3083147072 cites W2414283541 @default.
- W3083147072 cites W2552492585 @default.
- W3083147072 cites W2595624269 @default.
- W3083147072 cites W2595836338 @default.
- W3083147072 cites W2661309329 @default.
- W3083147072 cites W2743970427 @default.
- W3083147072 cites W2752322097 @default.
- W3083147072 cites W2886739398 @default.
- W3083147072 cites W2947797966 @default.
- W3083147072 cites W3011625057 @default.
- W3083147072 cites W3015494691 @default.
- W3083147072 doi "https://doi.org/10.1099/mic.0.000959" @default.
- W3083147072 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7660914" @default.
- W3083147072 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32886602" @default.
- W3083147072 hasPublicationYear "2020" @default.