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• W3083207478 endingPage "108216" @default.
• W3083207478 startingPage "108216" @default.
• W3083207478 abstract "N6-methyladenosine (m 6 A) is the most common post-transcriptional modification of RNA in eukaryotes that regulates the post-transcriptional expression level of genes without changing the base sequence . The role of m 6 A in fungal keratitis has not yet been elucidated. Here, we aimed to identify m 6 A modification changes and their potential roles in fungal keratitis. The murine model of fungal keratitis was established by inoculating mice with Fusarium solani ( F. solani ). The overall m 6 A level was detected via an m 6 A RNA methylation assay kit. The expression levels of key m 6 A modification-related genes were estimated by quantitative real-time polymerase chain reaction (PCR). The expression and localization of METTL (methyltransferase like)3, the key component of the m 6 A methyltransferase complex, was determined by immunostaining and Western blotting (WB). Immunoprecipitation methylation microarray was used to describe the changes in m 6 A modification in F. solani -infected corneal tissue. The overall m 6 A level in corneal tissue on the 5th day in the F. solani -treated group was upregulated compared with that in the control group. The demethylase levels were unaltered, but the level of the methylase METTL3 was increased significantly after fungal infection . Additionally, differences were found in m 6 A modifications in 1137 mRNAs, of which 780 were hypermethylated and 357 were hypomethylated. To the best of our knowledge, the present work is the first investigation on the m 6 A modification profiles in experimental fungal keratitis, and it may provide a potential therapeutic target. • 1. The overall m6A level in corneal tissue on the 5th day of F. solani -treated group was up-regulated. • 2. There were differences in m6A modifications at 1137 mRNAs, of which 780 were hypermethylated and 357 were hypomethylated. • 3. METTL3, was increased significantly at RNA and protein level after fungal infection." @default.
• W3083207478 created "2020-09-11" @default.
• W3083207478 creator A5000904990 @default.
• W3083207478 creator A5022634388 @default.
• W3083207478 date "2020-11-01" @default.
• W3083207478 modified "2023-09-25" @default.
• W3083207478 title "Fusarium infection alters the m6A-modified transcript landscape in the cornea" @default.
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• W3083207478 doi "https://doi.org/10.1016/j.exer.2020.108216" @default.
• W3083207478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32890482" @default.
• W3083207478 hasPublicationYear "2020" @default.
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