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• W3083536309 endingPage "e0238581" @default.
• W3083536309 startingPage "e0238581" @default.
• W3083536309 abstract "Electrowetting-on-dielectric is a decent technique to manipulate discrete volumes of liquid in form of droplets. In the last decade, electrowetting-on-dielectric systems, also called digital microfluidic systems, became more frequently used for a variety of applications because of their high flexibility and reconfigurability. Thus, one design can be adapted to different assays by only reprogramming. However, this flexibility can only be useful if the entire system is portable and easy to use. This paper presents the development of a portable, stand-alone digital microfluidic system based on a Linux-based operating system running on a Raspberry Pi, which is unique. We present PortaDrop exhibiting the following key features: (1) an all-in-one box approach, (2) a user-friendly, self-explaining graphical user interface and easy handling, (3) the ability of integrated electrochemical measurements, (4) the ease to implement additional lab equipment via Universal Serial Bus and the General Purpose Interface Bus as well as (5) a standardized experiment documentation. We propose that PortaDrop can be used to carry out experiments in different applications, where small sample volumes in the nanoliter to picoliter range need to be handled an analyzed automatically. As a first application, we present a protocol, where a droplet is consequently exchanged by droplets of another medium using passive dispensing. The exchange is monitored by electrical impedance spectroscopy. It is the first time, the media exchange caused by passive dispensing is characterized by electrochemical impedance spectroscopy. Summarizing, PortaDrop allows easy combination of fluid handling by means of electrowetting and additional sensing." @default.
• W3083536309 created "2020-09-11" @default.
• W3083536309 creator A5012143825 @default.
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• W3083536309 creator A5075028253 @default.
• W3083536309 creator A5077818031 @default.
• W3083536309 date "2020-09-03" @default.
• W3083536309 modified "2023-10-13" @default.
• W3083536309 title "PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications" @default.
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• W3083536309 doi "https://doi.org/10.1371/journal.pone.0238581" @default.
• W3083536309 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7470335" @default.
• W3083536309 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32881948" @default.
• W3083536309 hasPublicationYear "2020" @default.
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