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- W3083596246 endingPage "6551" @default.
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- W3083596246 abstract "Recent years have shown a tremendous increase and diversification in antibody-based therapeutics with advances in production techniques and formats. The plethora of currently investigated bi- to multi-specific antibody architectures can be harnessed to elicit a broad variety of specific modes of actions in oncology and immunology, spanning from enhanced selectivity to effector cell recruitment, all of which cannot be addressed by monospecific antibodies. Despite continuously growing efforts and methodologies, the identification of an optimal bispecific antibody as the best possible combination of two parental monospecific binders, however, remains challenging, due to tedious cloning and production, often resulting in undesired extended development times and increased expenses. Although automated high throughput screening approaches have matured for pharmaceutical small molecule development, it was only recently that protein bioconjugation technologies have been developed for the facile generation of bispecific antibodies in a ‘plug and play’ manner. In this review, we provide an overview of the most relevant methodologies for bispecific screening purposes—the DuoBody concept, paired light chain single cell production approaches, Sortase A and Transglutaminase, the SpyTag/SpyCatcher system, and inteins—and elaborate on the benefits as well as drawbacks of the different technologies." @default.
- W3083596246 created "2020-09-11" @default.
- W3083596246 creator A5002240767 @default.
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- W3083596246 creator A5029318279 @default.
- W3083596246 creator A5061836189 @default.
- W3083596246 creator A5063820128 @default.
- W3083596246 date "2020-09-08" @default.
- W3083596246 modified "2023-09-26" @default.
- W3083596246 title "Greatest Hits—Innovative Technologies for High Throughput Identification of Bispecific Antibodies" @default.
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