Matches in SemOpenAlex for { <https://semopenalex.org/work/W3083676186> ?p ?o ?g. }
- W3083676186 endingPage "201" @default.
- W3083676186 startingPage "196" @default.
- W3083676186 abstract "Cyclin D3 is a regulatory protein associated in a variety of human tumors. Despite several studies involving Cyclin D1 and D2, there are few articles that investigate the role of Cyclin D3. Therefore, this study aimed to produce and characterize Cyclin D3 using the Escherichia coli expression system and a protein refolding protocol. The anionic detergent SDS was used to solubilize the protein, then the solution were kept at 4 °C to precipitate SDS. After removing the precipitate by centrifugation, the supernatant was applied to the Ni-NTA column to purify His- tagged Cyclin D3. The recombinant protein shown to be refolded in a denaturation study by fluorescence spectroscopy at increasing concentrations of guanidine hydrochloride. The protein secondary structure, evaluated by circular dichroism, is composed of 39 % α helix and 15 % β-strands. In addition, the study aimed to validate the refolding protocol used to obtain Cyclin D3 from E. coli inclusion bodies." @default.
- W3083676186 created "2020-09-11" @default.
- W3083676186 creator A5002987701 @default.
- W3083676186 creator A5019463458 @default.
- W3083676186 creator A5031774799 @default.
- W3083676186 creator A5067051052 @default.
- W3083676186 creator A5075945387 @default.
- W3083676186 date "2020-12-01" @default.
- W3083676186 modified "2023-09-26" @default.
- W3083676186 title "Overexpression and refolding of human Cyclin D3. A reliable method or not?" @default.
- W3083676186 cites W1974343083 @default.
- W3083676186 cites W2004887889 @default.
- W3083676186 cites W2014144491 @default.
- W3083676186 cites W2014550271 @default.
- W3083676186 cites W2025938842 @default.
- W3083676186 cites W2026437584 @default.
- W3083676186 cites W2083780169 @default.
- W3083676186 cites W2089655089 @default.
- W3083676186 cites W2103519533 @default.
- W3083676186 cites W2138816428 @default.
- W3083676186 cites W2153492724 @default.
- W3083676186 cites W2398824588 @default.
- W3083676186 cites W2583136106 @default.
- W3083676186 cites W2607724955 @default.
- W3083676186 cites W2726920074 @default.
- W3083676186 cites W2902658589 @default.
- W3083676186 cites W2983594457 @default.
- W3083676186 cites W3004233517 @default.
- W3083676186 cites W36700065 @default.
- W3083676186 cites W4245617670 @default.
- W3083676186 doi "https://doi.org/10.1016/j.procbio.2020.09.004" @default.
- W3083676186 hasPublicationYear "2020" @default.
- W3083676186 type Work @default.
- W3083676186 sameAs 3083676186 @default.
- W3083676186 citedByCount "0" @default.
- W3083676186 crossrefType "journal-article" @default.
- W3083676186 hasAuthorship W3083676186A5002987701 @default.
- W3083676186 hasAuthorship W3083676186A5019463458 @default.
- W3083676186 hasAuthorship W3083676186A5031774799 @default.
- W3083676186 hasAuthorship W3083676186A5067051052 @default.
- W3083676186 hasAuthorship W3083676186A5075945387 @default.
- W3083676186 hasConcept C104317684 @default.
- W3083676186 hasConcept C11882975 @default.
- W3083676186 hasConcept C120089663 @default.
- W3083676186 hasConcept C12554922 @default.
- W3083676186 hasConcept C133571119 @default.
- W3083676186 hasConcept C13965031 @default.
- W3083676186 hasConcept C1491633281 @default.
- W3083676186 hasConcept C153911025 @default.
- W3083676186 hasConcept C16438837 @default.
- W3083676186 hasConcept C185592680 @default.
- W3083676186 hasConcept C199835354 @default.
- W3083676186 hasConcept C2776923230 @default.
- W3083676186 hasConcept C2779463914 @default.
- W3083676186 hasConcept C2781403221 @default.
- W3083676186 hasConcept C28179978 @default.
- W3083676186 hasConcept C29537977 @default.
- W3083676186 hasConcept C40767141 @default.
- W3083676186 hasConcept C43617362 @default.
- W3083676186 hasConcept C547475151 @default.
- W3083676186 hasConcept C55493867 @default.
- W3083676186 hasConcept C7623868 @default.
- W3083676186 hasConcept C86803240 @default.
- W3083676186 hasConcept C9743823 @default.
- W3083676186 hasConceptScore W3083676186C104317684 @default.
- W3083676186 hasConceptScore W3083676186C11882975 @default.
- W3083676186 hasConceptScore W3083676186C120089663 @default.
- W3083676186 hasConceptScore W3083676186C12554922 @default.
- W3083676186 hasConceptScore W3083676186C133571119 @default.
- W3083676186 hasConceptScore W3083676186C13965031 @default.
- W3083676186 hasConceptScore W3083676186C1491633281 @default.
- W3083676186 hasConceptScore W3083676186C153911025 @default.
- W3083676186 hasConceptScore W3083676186C16438837 @default.
- W3083676186 hasConceptScore W3083676186C185592680 @default.
- W3083676186 hasConceptScore W3083676186C199835354 @default.
- W3083676186 hasConceptScore W3083676186C2776923230 @default.
- W3083676186 hasConceptScore W3083676186C2779463914 @default.
- W3083676186 hasConceptScore W3083676186C2781403221 @default.
- W3083676186 hasConceptScore W3083676186C28179978 @default.
- W3083676186 hasConceptScore W3083676186C29537977 @default.
- W3083676186 hasConceptScore W3083676186C40767141 @default.
- W3083676186 hasConceptScore W3083676186C43617362 @default.
- W3083676186 hasConceptScore W3083676186C547475151 @default.
- W3083676186 hasConceptScore W3083676186C55493867 @default.
- W3083676186 hasConceptScore W3083676186C7623868 @default.
- W3083676186 hasConceptScore W3083676186C86803240 @default.
- W3083676186 hasConceptScore W3083676186C9743823 @default.
- W3083676186 hasFunder F4320320997 @default.
- W3083676186 hasFunder F4320321091 @default.
- W3083676186 hasLocation W30836761861 @default.
- W3083676186 hasOpenAccess W3083676186 @default.
- W3083676186 hasPrimaryLocation W30836761861 @default.
- W3083676186 hasRelatedWork W128824071 @default.
- W3083676186 hasRelatedWork W1507530325 @default.
- W3083676186 hasRelatedWork W1977340592 @default.
- W3083676186 hasRelatedWork W1982298196 @default.
- W3083676186 hasRelatedWork W2039496237 @default.
- W3083676186 hasRelatedWork W2049158425 @default.