FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3083700512> ?p ?o ?g. }

• W3083700512 endingPage "129725" @default.
• W3083700512 startingPage "129725" @default.
• W3083700512 abstract "DNA bridging promoted by the H-NS protein, combined with the compaction induced by cellular crowding, plays a major role in the structuring of the E. coli genome. However, only few studies consider the effects of the physical interplay of these two factors in a controlled environment. We apply a single molecule technique (Magnetic Tweezers) to study the nanomechanics of compaction and folding kinetics of a 6 kb DNA fragment, induced by H-NS bridging and/or PEG crowding. In the presence of H-NS alone, the DNA shows a step-wise collapse driven by the formation of multiple bridges, and little variations in the H-NS concentration-dependent unfolding force. Conversely, the DNA collapse force observed with PEG was highly dependent on the volume fraction of the crowding agent. The two limit cases were interpreted considering the models of loop formation in a pulled chain and pulling of an equilibrium globule respectively. We observed an evident cooperative effect between H-NS activity and the depletion of forces induced by PEG. Our data suggest a double role for H-NS in enhancing compaction while forming specific loops, which could be crucial in vivo for defining specific mesoscale domains in chromosomal regions in response to environmental changes." @default.
• W3083700512 created "2020-09-11" @default.
• W3083700512 creator A5002704035 @default.
• W3083700512 creator A5010329445 @default.
• W3083700512 creator A5018255205 @default.
• W3083700512 creator A5019466584 @default.
• W3083700512 creator A5041383409 @default.
• W3083700512 creator A5041638323 @default.
• W3083700512 creator A5050873610 @default.
• W3083700512 creator A5058923795 @default.
• W3083700512 creator A5065480824 @default.
• W3083700512 creator A5079589588 @default.
• W3083700512 date "2020-12-01" @default.
• W3083700512 modified "2023-09-30" @default.
• W3083700512 title "Cooperative effects on the compaction of DNA fragments by the nucleoid protein H-NS and the crowding agent PEG probed by Magnetic Tweezers" @default.
• W3083700512 cites W1659688442 @default.
• W3083700512 cites W1953757982 @default.
• W3083700512 cites W1966913029 @default.
• W3083700512 cites W1967639299 @default.
• W3083700512 cites W1971760608 @default.
• W3083700512 cites W1974600199 @default.
• W3083700512 cites W1976122370 @default.
• W3083700512 cites W1977998168 @default.
• W3083700512 cites W1985349755 @default.
• W3083700512 cites W1985963323 @default.
• W3083700512 cites W1987697568 @default.
• W3083700512 cites W1998276013 @default.
• W3083700512 cites W1998897210 @default.
• W3083700512 cites W2002645780 @default.
• W3083700512 cites W2016160555 @default.
• W3083700512 cites W2018775916 @default.
• W3083700512 cites W2028488968 @default.
• W3083700512 cites W2033877647 @default.
• W3083700512 cites W2036475903 @default.
• W3083700512 cites W2037321511 @default.
• W3083700512 cites W2042933003 @default.
• W3083700512 cites W2045696781 @default.
• W3083700512 cites W2045939673 @default.
• W3083700512 cites W2048653869 @default.
• W3083700512 cites W2058946062 @default.
• W3083700512 cites W2060475454 @default.
• W3083700512 cites W2062139873 @default.
• W3083700512 cites W2063649341 @default.
• W3083700512 cites W2069138165 @default.
• W3083700512 cites W2073169516 @default.
• W3083700512 cites W2079518832 @default.
• W3083700512 cites W2081531625 @default.
• W3083700512 cites W2081749061 @default.
• W3083700512 cites W2083278118 @default.
• W3083700512 cites W2086142065 @default.
• W3083700512 cites W2089618969 @default.
• W3083700512 cites W2096926750 @default.
• W3083700512 cites W2109811392 @default.
• W3083700512 cites W2111662566 @default.
• W3083700512 cites W2113843263 @default.
• W3083700512 cites W2123119094 @default.
• W3083700512 cites W2126464107 @default.
• W3083700512 cites W2127552033 @default.
• W3083700512 cites W2136440044 @default.
• W3083700512 cites W2136955543 @default.
• W3083700512 cites W2148570085 @default.
• W3083700512 cites W2149366594 @default.
• W3083700512 cites W2150702427 @default.
• W3083700512 cites W2150766477 @default.
• W3083700512 cites W2152763121 @default.
• W3083700512 cites W2152919627 @default.
• W3083700512 cites W2154407985 @default.
• W3083700512 cites W2158208080 @default.
• W3083700512 cites W2160174395 @default.
• W3083700512 cites W2161118206 @default.
• W3083700512 cites W2161435888 @default.
• W3083700512 cites W2163558891 @default.
• W3083700512 cites W2252628946 @default.
• W3083700512 cites W2261995446 @default.
• W3083700512 cites W2266731155 @default.
• W3083700512 cites W2292512187 @default.
• W3083700512 cites W2327423361 @default.
• W3083700512 cites W2338378533 @default.
• W3083700512 cites W2338940958 @default.
• W3083700512 cites W2461989827 @default.
• W3083700512 cites W2553373724 @default.
• W3083700512 cites W2558879079 @default.
• W3083700512 cites W2604185808 @default.
• W3083700512 cites W2616075393 @default.
• W3083700512 cites W2767381186 @default.
• W3083700512 cites W2791638215 @default.
• W3083700512 cites W2800222644 @default.
• W3083700512 cites W2891385000 @default.
• W3083700512 cites W2900007277 @default.
• W3083700512 cites W2912166035 @default.
• W3083700512 cites W3001479080 @default.
• W3083700512 cites W3103518005 @default.
• W3083700512 cites W3106340622 @default.
• W3083700512 cites W4234689925 @default.
• W3083700512 doi "https://doi.org/10.1016/j.bbagen.2020.129725" @default.
• W3083700512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32891648" @default.
• W3083700512 hasPublicationYear "2020" @default.
• W3083700512 type Work @default.

• next