FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3083730033> ?p ?o ?g. }

• W3083730033 abstract "Oral squamous cell carcinoma (OSCC) is a major cancer type worldwide, ranked in among top ten cancers. However, in South Asia, OSCC is at third position in commonly occurring cancer. Pakistan is ranked at tenth level in world but second in South Asia in OSCC. High ratio of cancer suggests that it is a major reason of death around the globe and is mainly because of the late-stage diagnosis. Metastasis of OSCC depends on many environmental and genetic factors including different genes, proteins and non-coding RNAs i.e. miRNAs. In cancer cells, miRNAs express themselves differentially. This differential expression of miRNAs in cancer cells can be explored and is worth considering as a diagnostic biomarker. On account of these facts, this study was designed to aim at detection of miRNAs and their target genes in OSCC through functional and expression analysis. We already have reported a list of miRNAs and their differentially expressed target genes in OSCC along with their functional and network analysis, in an in silico study. To evaluate the role of miRNAs and their target genes in OSCC patients of Pakistan, we measured the expression of four miRNAs; miR-300, miR-27a-3p, miR-340-5p, miR-205-3p with their corresponding target genes; ALB, CDH1, CACNA1, ARID5B, CDKN1A, BCL2, ABL1 in saliva samples of patients (n=100) and healthy controls (n=100). Total RNA was extracted from saliva samples; cDNA was synthesized and real time quantitative PCR was used to measure the expression. Preliminary results showed that miRNAs were down regulated in saliva samples of patients as compared to healthy controls. Consequently, target genes of each miRNA were dysregulated in patients. miR-300 targets CDH-1 and ALB and there was a direct relationship of miR-300 with ALB. Both of them were down regulated in OSCC patients. We found a down regulation in the expression of miRNA-27a-3p and its target genes CACNA1I and ARID5B in the saliva samples of patients when compared with the healthy controls. This suggests that there is a direct relationship between the miRNA-27a-3p and its target genes CACNA1I and ARID5B. We identified that miRNA-340-5p targets BCL2 and CDKN1A genes; miR-340-5p was down regulated by targeting both genes and also down regulated their expression, which results in OSCC. miRNA-205-3p targets ABL1 gene and there is direct relationship between the two. Both of them were down regulated in OSCC patients and change in expression leads to OSCC. The change in expression pattern of miRNAs and their target genes suggests a potential diagnostic role of these miRNAs in early detection of OSCC by saliva samples at early stages. Citation Format: Muhammad Jawad Khan, Andleeb Zahra, Ghulam Rabia, Syeda Aba Ali, Shanza Sahar, Sobia Bibi, M. Qaiser Fatmi. Role of salivary miRNAs and their target genes in oral cancer patients as a potential diagnostic tool [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 3700." @default.
• W3083730033 created "2020-09-11" @default.
• W3083730033 creator A5022876559 @default.
• W3083730033 creator A5034762845 @default.
• W3083730033 creator A5040488354 @default.
• W3083730033 creator A5046022169 @default.
• W3083730033 creator A5058482004 @default.
• W3083730033 creator A5078032577 @default.
• W3083730033 creator A5090556896 @default.
• W3083730033 date "2020-08-13" @default.
• W3083730033 modified "2023-10-18" @default.
• W3083730033 title "Abstract 3700: Role of salivary miRNAs and their target genes in oral cancer patients as a potential diagnostic tool" @default.
• W3083730033 doi "https://doi.org/10.1158/1538-7445.am2020-3700" @default.
• W3083730033 hasPublicationYear "2020" @default.
• W3083730033 type Work @default.
• W3083730033 sameAs 3083730033 @default.
• W3083730033 citedByCount "0" @default.
• W3083730033 crossrefType "proceedings-article" @default.
• W3083730033 hasAuthorship W3083730033A5022876559 @default.
• W3083730033 hasAuthorship W3083730033A5034762845 @default.
• W3083730033 hasAuthorship W3083730033A5040488354 @default.
• W3083730033 hasAuthorship W3083730033A5046022169 @default.
• W3083730033 hasAuthorship W3083730033A5058482004 @default.
• W3083730033 hasAuthorship W3083730033A5078032577 @default.
• W3083730033 hasAuthorship W3083730033A5090556896 @default.
• W3083730033 hasConcept C104317684 @default.
• W3083730033 hasConcept C121608353 @default.
• W3083730033 hasConcept C145059251 @default.
• W3083730033 hasConcept C1491633281 @default.
• W3083730033 hasConcept C149402561 @default.
• W3083730033 hasConcept C2776391639 @default.
• W3083730033 hasConcept C2779013556 @default.
• W3083730033 hasConcept C2781197716 @default.
• W3083730033 hasConcept C502942594 @default.
• W3083730033 hasConcept C504460877 @default.
• W3083730033 hasConcept C54355233 @default.
• W3083730033 hasConcept C55493867 @default.
• W3083730033 hasConcept C60644358 @default.
• W3083730033 hasConcept C70721500 @default.
• W3083730033 hasConcept C86803240 @default.
• W3083730033 hasConceptScore W3083730033C104317684 @default.
• W3083730033 hasConceptScore W3083730033C121608353 @default.
• W3083730033 hasConceptScore W3083730033C145059251 @default.
• W3083730033 hasConceptScore W3083730033C1491633281 @default.
• W3083730033 hasConceptScore W3083730033C149402561 @default.
• W3083730033 hasConceptScore W3083730033C2776391639 @default.
• W3083730033 hasConceptScore W3083730033C2779013556 @default.
• W3083730033 hasConceptScore W3083730033C2781197716 @default.
• W3083730033 hasConceptScore W3083730033C502942594 @default.
• W3083730033 hasConceptScore W3083730033C504460877 @default.
• W3083730033 hasConceptScore W3083730033C54355233 @default.
• W3083730033 hasConceptScore W3083730033C55493867 @default.
• W3083730033 hasConceptScore W3083730033C60644358 @default.
• W3083730033 hasConceptScore W3083730033C70721500 @default.
• W3083730033 hasConceptScore W3083730033C86803240 @default.
• W3083730033 hasLocation W30837300331 @default.
• W3083730033 hasOpenAccess W3083730033 @default.
• W3083730033 hasPrimaryLocation W30837300331 @default.
• W3083730033 hasRelatedWork W11792359 @default.
• W3083730033 hasRelatedWork W12740073 @default.
• W3083730033 hasRelatedWork W13804785 @default.
• W3083730033 hasRelatedWork W20775107 @default.
• W3083730033 hasRelatedWork W22582601 @default.
• W3083730033 hasRelatedWork W26061940 @default.
• W3083730033 hasRelatedWork W27958817 @default.
• W3083730033 hasRelatedWork W28857407 @default.
• W3083730033 hasRelatedWork W29693034 @default.
• W3083730033 hasRelatedWork W37155516 @default.
• W3083730033 isParatext "false" @default.
• W3083730033 isRetracted "false" @default.
• W3083730033 magId "3083730033" @default.
• W3083730033 workType "article" @default.