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- W3083873810 abstract "Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high– or low–/non–IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL." @default.
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- W3083873810 date "2020-12-02" @default.
- W3083873810 modified "2023-10-06" @default.
- W3083873810 title "Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis" @default.
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- W3083873810 doi "https://doi.org/10.4269/ajtmh.20-0124" @default.
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