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• W3083921400 abstract "Abstract We set up a Cae norhabditis elegans model to extrapolate anthelmintic (AH) effects of commercial polyphenols to related gastrointestinal zoonotic species. We compared the practical convenience of S liquid to solid agar Nematode grown medium in daily reproduction patterns and total brood size. Solid agar resulted a highly effective, reproducibly medium, in a less costly and rapid time manner. A minimum of three replications per monomer concentration are suggested to get a robust statistical analysis. Flavonoids Quercetin and Rutin potentially decrease brood sizes and percentage of development, with the exception of 20μM concentration. Flavanols resulted heterogeneous. In a dose-dependent manner Catechin 20μM significantly decreased egg output, and egg hatching in a 15% on respect to control values. Contrary, Epigallocatechin-gallate, seems to stimulate brood sizes and egg-hatching, however, EGCG10μM decreased reproduction but with no in a significative manner. We found a nematocidal effect on the reproductive parameters of C. elegans exposed to the chalcones Phloretin and Phloridzin, and Flavones Flavone and Genistein. A significant general decrease on reproductive parameters were found, particularly significant for Genistein 20μM in the percentage of hatching respect to control. Effects of time schedule and monomer concentration were found for the Hydrolysable tannins Gallic and Tannic acid. Tannic acid showed effectiveness against egg-hatching. The spectrum of percentages of change compared to each blank resulted negative decreasing fertility values, but for GA5, associated with a net increase of larvae hatched. 1 AH, GIN, CTS, HTs Q) Quercetin, (R) Rutin, (C) (+)-Catechin hydrate, (EGCG) (-)-Epigallocatechin Gallate, (PTN) Phloretin, (PDN) Phloridzin Dihydrate, (GE) Genistein, (FLA) Flavone, (GA) Gallic acid and (TA) Tannic acid., G1 (adult worm originally seeded per plate and monomer concentration at time 0. Two replications were performed per plate). Solvents: PBS (Phosphate Buffer Solution), CH4O (Methanol 0,002%) L3" @default.
• W3083921400 created "2020-09-14" @default.
• W3083921400 creator A5038125100 @default.
• W3083921400 date "2020-09-10" @default.
• W3083921400 modified "2023-09-25" @default.
• W3083921400 title "<i>Caenorhabditis elegans</i>: a model to study the anthelmintic effects of polyphenolic compounds on the fertility of parasitic gastrointestinal nematodes?" @default.
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• W3083921400 doi "https://doi.org/10.1101/2020.09.09.289637" @default.
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