Matches in SemOpenAlex for { <https://semopenalex.org/work/W3084007385> ?p ?o ?g. }
- W3084007385 endingPage "1201" @default.
- W3084007385 startingPage "1191" @default.
- W3084007385 abstract "Porcine reproductive and respiratory syndrome virus (PRRSV) causes an economically important global swine disease. Here we report the development of subunit PRRSV-2 vaccines by expressing swine leucocyte antigen (SLA) class I and class II allele-specific epitope antigens in a robust adenovirus vector. SLA I-specific CD8 and SLA II-specific CD4 T cell epitopes of PRRSV-2 NADC20 were predicted in silico . Stable murine leukaemia cell lines (RMA-S), which are TAP-deficient and lacking endogenous class I epitope loading, were established to express different SLA I alleles. The binding stability of PRRSV T cell epitope peptides with SLA I alleles expressed on RMA-S cells was characterized. Two PRRSV poly-T cell epitope peptides were designed. NADC20-PP1 included 39 class I epitopes, consisting of 8 top-ranked epitopes specific to each of 5 SLA I alleles, and fused to 5 class II epitopes specific to SLA II alleles. NADC20-PP2, a subset of PP1, included two top-ranked class I epitopes specific to each of the five SLA I alleles. Two vaccine candidates, Ad-NADC20-PP1 and Ad-NADC20-PP2, were constructed by expressing the polytope peptides in a replication-incompetent human adenovirus 5 vector. A vaccination and challenge study in 30 piglets showed that animals vaccinated with the vaccines had numerically lower gross and histopathology lung lesions, and numerically lower PRRSV RNA loads in lung and serum after challenge compared to the controls, although there was no statistical significance. The results suggested that the Ad-NADC20-PP1 and Ad-NADC20-PP2 vaccines provided little or no protection, further highlighting the tremendous challenges faced in developing an effective subunit PRRSV-2 vaccine." @default.
- W3084007385 created "2020-09-14" @default.
- W3084007385 creator A5001163781 @default.
- W3084007385 creator A5019671434 @default.
- W3084007385 creator A5028425506 @default.
- W3084007385 creator A5029039108 @default.
- W3084007385 creator A5030690206 @default.
- W3084007385 creator A5037600391 @default.
- W3084007385 creator A5037652993 @default.
- W3084007385 creator A5039743180 @default.
- W3084007385 creator A5056969824 @default.
- W3084007385 creator A5075780100 @default.
- W3084007385 creator A5077928113 @default.
- W3084007385 creator A5079021767 @default.
- W3084007385 date "2020-11-01" @default.
- W3084007385 modified "2023-10-14" @default.
- W3084007385 title "Construction and efficacy evaluation of novel swine leukocyte antigen (SLA) class I and class II allele-specific poly-T cell epitope vaccines against porcine reproductive and respiratory syndrome virus" @default.
- W3084007385 cites W1964565857 @default.
- W3084007385 cites W1975109518 @default.
- W3084007385 cites W1978538643 @default.
- W3084007385 cites W1979842474 @default.
- W3084007385 cites W1984295177 @default.
- W3084007385 cites W1984573803 @default.
- W3084007385 cites W1998128231 @default.
- W3084007385 cites W2006629564 @default.
- W3084007385 cites W2014689076 @default.
- W3084007385 cites W2019542752 @default.
- W3084007385 cites W2021084311 @default.
- W3084007385 cites W2025031008 @default.
- W3084007385 cites W2032224519 @default.
- W3084007385 cites W2033120087 @default.
- W3084007385 cites W2067278738 @default.
- W3084007385 cites W2078533322 @default.
- W3084007385 cites W2084282306 @default.
- W3084007385 cites W2088526109 @default.
- W3084007385 cites W2093606554 @default.
- W3084007385 cites W2103692755 @default.
- W3084007385 cites W2105387318 @default.
- W3084007385 cites W2134759677 @default.
- W3084007385 cites W2138124339 @default.
- W3084007385 cites W2140296277 @default.
- W3084007385 cites W2142467502 @default.
- W3084007385 cites W2168254025 @default.
- W3084007385 cites W2171170003 @default.
- W3084007385 cites W2174353003 @default.
- W3084007385 cites W2176272660 @default.
- W3084007385 cites W2260159930 @default.
- W3084007385 cites W232721668 @default.
- W3084007385 cites W2564714357 @default.
- W3084007385 cites W2600196521 @default.
- W3084007385 cites W2613325709 @default.
- W3084007385 cites W2766897332 @default.
- W3084007385 cites W2773464652 @default.
- W3084007385 cites W2776411534 @default.
- W3084007385 cites W2974473741 @default.
- W3084007385 cites W2989159428 @default.
- W3084007385 cites W4238945854 @default.
- W3084007385 cites W755303132 @default.
- W3084007385 cites W970789458 @default.
- W3084007385 doi "https://doi.org/10.1099/jgv.0.001492" @default.
- W3084007385 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32894211" @default.
- W3084007385 hasPublicationYear "2020" @default.
- W3084007385 type Work @default.
- W3084007385 sameAs 3084007385 @default.
- W3084007385 citedByCount "4" @default.
- W3084007385 countsByYear W30840073852021 @default.
- W3084007385 countsByYear W30840073852023 @default.
- W3084007385 crossrefType "journal-article" @default.
- W3084007385 hasAuthorship W3084007385A5001163781 @default.
- W3084007385 hasAuthorship W3084007385A5019671434 @default.
- W3084007385 hasAuthorship W3084007385A5028425506 @default.
- W3084007385 hasAuthorship W3084007385A5029039108 @default.
- W3084007385 hasAuthorship W3084007385A5030690206 @default.
- W3084007385 hasAuthorship W3084007385A5037600391 @default.
- W3084007385 hasAuthorship W3084007385A5037652993 @default.
- W3084007385 hasAuthorship W3084007385A5039743180 @default.
- W3084007385 hasAuthorship W3084007385A5056969824 @default.
- W3084007385 hasAuthorship W3084007385A5075780100 @default.
- W3084007385 hasAuthorship W3084007385A5077928113 @default.
- W3084007385 hasAuthorship W3084007385A5079021767 @default.
- W3084007385 hasBestOaLocation W30840073851 @default.
- W3084007385 hasConcept C104317684 @default.
- W3084007385 hasConcept C147483822 @default.
- W3084007385 hasConcept C159047783 @default.
- W3084007385 hasConcept C167672396 @default.
- W3084007385 hasConcept C170627219 @default.
- W3084007385 hasConcept C180754005 @default.
- W3084007385 hasConcept C195616568 @default.
- W3084007385 hasConcept C203014093 @default.
- W3084007385 hasConcept C2522874641 @default.
- W3084007385 hasConcept C2779819720 @default.
- W3084007385 hasConcept C54355233 @default.
- W3084007385 hasConcept C86803240 @default.
- W3084007385 hasConceptScore W3084007385C104317684 @default.
- W3084007385 hasConceptScore W3084007385C147483822 @default.
- W3084007385 hasConceptScore W3084007385C159047783 @default.
- W3084007385 hasConceptScore W3084007385C167672396 @default.
- W3084007385 hasConceptScore W3084007385C170627219 @default.