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- W3084129619 endingPage "20200350" @default.
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- W3084129619 abstract "Spore-forming bacteria modulate their metabolic rate by over five orders of magnitude as they transition between dormant spores and vegetative cells and thus represent an extreme case of phenotypic variation. During environmental changes in nutrient availability, clonal populations of spore-forming bacteria exhibit individual differences in cell fate, the timing of phenotypic transitions and gene expression. One potential source of this variability is metabolic heterogeneity, but this has not yet been measured, as existing single-cell methods are not easily applicable to spores due to their small size and strong autofluorescence. Here, we use the bacterial bioluminescence system and a highly sensitive microscope to measure metabolic dynamics in thousands of B. subtilis spores as they germinate. We observe and quantitate large variations in the bioluminescence dynamics across individual spores that can be decomposed into contributions from variability in germination timing, the amount of endogenously produced luminescence substrate and the intracellular reducing power. This work shows that quantitative measurement of spore metabolism is possible and thus it opens avenues for future study of the thermodynamic nature of dormant states." @default.
- W3084129619 created "2020-09-14" @default.
- W3084129619 creator A5050002665 @default.
- W3084129619 creator A5057840680 @default.
- W3084129619 date "2020-09-01" @default.
- W3084129619 modified "2023-10-10" @default.
- W3084129619 title "Bioluminescence dynamics in single germinating bacterial spores reveal metabolic heterogeneity" @default.
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- W3084129619 doi "https://doi.org/10.1098/rsif.2020.0350" @default.
- W3084129619 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7536063" @default.
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