Matches in SemOpenAlex for { <https://semopenalex.org/work/W308482606> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W308482606 endingPage "215" @default.
- W308482606 startingPage "209" @default.
- W308482606 abstract "【Objective】 In this study, the andropin gene of drosophila was cloned into Escherichia coli to produce high activity antibacterial peptide by prokaryotic expression system. 【Method】 Two sequences and a pair of primers of the Drosophila Andropin gene were designed and synthesized by chemical method and the whole Andropin sequence was obtained by complementary overlay area PCR method. A recombination fusion expressing vector pET32a-Anp was constructed and transformed into an E. coli host named OrigammiB. The expressed recombinant protein was purified by His-tag Purification kit, digested by enterokinase, and then its antibacterial activity was tested by agar diffusion assay. 【Result】 The result showed that the Andropin was expressed as a fusion protein in a 70% soluble form with a theoretical molecular weight of 28ku. Following induction under the condition of 1mmol/L IPTG for 4 h at 37℃, the high-level expression of the fusion protein can be achieved and the amount of the fusion protein reached 30% of the total proteins. The activity assay demonstrated that the peptide had active ability against the E. coli and Staphylococcus aureaus. 【Conclusion】 The recombinant engineered bacteria expressed the Andropin fusion peptide and activity assay demonstrated that the peptide had antibacterial biological activity." @default.
- W308482606 created "2016-06-24" @default.
- W308482606 creator A5038660647 @default.
- W308482606 creator A5041603258 @default.
- W308482606 creator A5045423223 @default.
- W308482606 creator A5075342871 @default.
- W308482606 date "2010-07-10" @default.
- W308482606 modified "2023-09-27" @default.
- W308482606 title "果蝇抗菌肽基因(Andropin)的原核表达与活性分析" @default.
- W308482606 hasPublicationYear "2010" @default.
- W308482606 type Work @default.
- W308482606 sameAs 308482606 @default.
- W308482606 citedByCount "0" @default.
- W308482606 crossrefType "journal-article" @default.
- W308482606 hasAuthorship W308482606A5038660647 @default.
- W308482606 hasAuthorship W308482606A5041603258 @default.
- W308482606 hasAuthorship W308482606A5045423223 @default.
- W308482606 hasAuthorship W308482606A5075342871 @default.
- W308482606 hasConcept C104317684 @default.
- W308482606 hasConcept C111829193 @default.
- W308482606 hasConcept C123894998 @default.
- W308482606 hasConcept C145508813 @default.
- W308482606 hasConcept C153911025 @default.
- W308482606 hasConcept C170344550 @default.
- W308482606 hasConcept C185592680 @default.
- W308482606 hasConcept C2779281246 @default.
- W308482606 hasConcept C2780104969 @default.
- W308482606 hasConcept C33161422 @default.
- W308482606 hasConcept C40767141 @default.
- W308482606 hasConcept C523546767 @default.
- W308482606 hasConcept C54355233 @default.
- W308482606 hasConcept C547475151 @default.
- W308482606 hasConcept C55493867 @default.
- W308482606 hasConcept C86803240 @default.
- W308482606 hasConceptScore W308482606C104317684 @default.
- W308482606 hasConceptScore W308482606C111829193 @default.
- W308482606 hasConceptScore W308482606C123894998 @default.
- W308482606 hasConceptScore W308482606C145508813 @default.
- W308482606 hasConceptScore W308482606C153911025 @default.
- W308482606 hasConceptScore W308482606C170344550 @default.
- W308482606 hasConceptScore W308482606C185592680 @default.
- W308482606 hasConceptScore W308482606C2779281246 @default.
- W308482606 hasConceptScore W308482606C2780104969 @default.
- W308482606 hasConceptScore W308482606C33161422 @default.
- W308482606 hasConceptScore W308482606C40767141 @default.
- W308482606 hasConceptScore W308482606C523546767 @default.
- W308482606 hasConceptScore W308482606C54355233 @default.
- W308482606 hasConceptScore W308482606C547475151 @default.
- W308482606 hasConceptScore W308482606C55493867 @default.
- W308482606 hasConceptScore W308482606C86803240 @default.
- W308482606 hasIssue "7" @default.
- W308482606 hasLocation W3084826061 @default.
- W308482606 hasOpenAccess W308482606 @default.
- W308482606 hasPrimaryLocation W3084826061 @default.
- W308482606 hasVolume "38" @default.
- W308482606 isParatext "false" @default.
- W308482606 isRetracted "false" @default.
- W308482606 magId "308482606" @default.
- W308482606 workType "article" @default.