FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3085292324> ?p ?o ?g. }

• W3085292324 endingPage "2244" @default.
• W3085292324 startingPage "2226" @default.
• W3085292324 abstract "Identification of the molecular networks that facilitated the evolution of multicellular animals from their unicellular ancestors is a fundamental problem in evolutionary cellular biology. Choanoflagellates are recognized as the closest extant nonmetazoan ancestors to animals. These unicellular eukaryotes can adopt a multicellular-like rosette state. Therefore, they are compelling models for the study of early multicellularity. Comparative studies revealed that a number of putative human orthologs are present in choanoflagellate genomes, suggesting that a subset of these genes were necessary for the emergence of multicellularity. However, previous work is largely based on sequence alignments alone, which does not confirm structural nor functional similarity. Here, we focus on the PDZ domain, a peptide-binding domain which plays critical roles in myriad cellular signaling networks and which underwent a gene family expansion in metazoan lineages. Using a customized sequence similarity search algorithm, we identified 178 PDZ domains in the Monosiga brevicollis proteome. This includes 11 previously unidentified sequences, which we analyzed using Rosetta and homology modeling. To assess conservation of protein structure, we solved high-resolution crystal structures of representative M. brevicollis PDZ domains that are homologous to human Dlg1 PDZ2, Dlg1 PDZ3, GIPC, and SHANK1 PDZ domains. To assess functional conservation, we calculated binding affinities for mbGIPC, mbSHANK1, mbSNX27, and mbDLG-3 PDZ domains from M. brevicollis. Overall, we find that peptide selectivity is generally conserved between these two disparate organisms, with one possible exception, mbDLG-3. Overall, our results provide novel insight into signaling pathways in a choanoflagellate model of primitive multicellularity." @default.
• W3085292324 created "2020-09-21" @default.
• W3085292324 creator A5004159284 @default.
• W3085292324 creator A5017834214 @default.
• W3085292324 creator A5021321754 @default.
• W3085292324 creator A5033745454 @default.
• W3085292324 creator A5050379643 @default.
• W3085292324 creator A5052630353 @default.
• W3085292324 creator A5057399557 @default.
• W3085292324 creator A5064481407 @default.
• W3085292324 creator A5080403123 @default.
• W3085292324 creator A5081259787 @default.
• W3085292324 creator A5088817733 @default.
• W3085292324 date "2020-09-25" @default.
• W3085292324 modified "2023-10-14" @default.
• W3085292324 title "Structural characterization and computational analysis of <scp>PDZ</scp> domains in <i>Monosiga brevicollis</i>" @default.
• W3085292324 cites W1724277458 @default.
• W3085292324 cites W177303547 @default.
• W3085292324 cites W1897589155 @default.
• W3085292324 cites W1967194316 @default.
• W3085292324 cites W1970186847 @default.
• W3085292324 cites W1983820999 @default.
• W3085292324 cites W1987474460 @default.
• W3085292324 cites W1994996237 @default.
• W3085292324 cites W1995808589 @default.
• W3085292324 cites W1997209552 @default.
• W3085292324 cites W2000251153 @default.
• W3085292324 cites W2000840258 @default.
• W3085292324 cites W2007399351 @default.
• W3085292324 cites W2009010596 @default.
• W3085292324 cites W2016255772 @default.
• W3085292324 cites W2025601221 @default.
• W3085292324 cites W2026582002 @default.
• W3085292324 cites W2029440564 @default.
• W3085292324 cites W2031407920 @default.
• W3085292324 cites W2032574536 @default.
• W3085292324 cites W2034639396 @default.
• W3085292324 cites W2043083737 @default.
• W3085292324 cites W2048000913 @default.
• W3085292324 cites W2054239223 @default.
• W3085292324 cites W2055043387 @default.
• W3085292324 cites W2063156579 @default.
• W3085292324 cites W2063216482 @default.
• W3085292324 cites W2065027762 @default.
• W3085292324 cites W2065989745 @default.
• W3085292324 cites W2071430620 @default.
• W3085292324 cites W2073360590 @default.
• W3085292324 cites W2073456836 @default.
• W3085292324 cites W2074570440 @default.
• W3085292324 cites W2082147709 @default.
• W3085292324 cites W2083178779 @default.
• W3085292324 cites W2086549128 @default.
• W3085292324 cites W2088028188 @default.
• W3085292324 cites W2088239473 @default.
• W3085292324 cites W2088365719 @default.
• W3085292324 cites W2092220359 @default.
• W3085292324 cites W2095036253 @default.
• W3085292324 cites W2098565607 @default.
• W3085292324 cites W2114559206 @default.
• W3085292324 cites W2115167726 @default.
• W3085292324 cites W2115291348 @default.
• W3085292324 cites W2116423958 @default.
• W3085292324 cites W2121937133 @default.
• W3085292324 cites W2122997762 @default.
• W3085292324 cites W2124026197 @default.
• W3085292324 cites W2124829726 @default.
• W3085292324 cites W2125254321 @default.
• W3085292324 cites W2127130852 @default.
• W3085292324 cites W2127325055 @default.
• W3085292324 cites W2129130876 @default.
• W3085292324 cites W2130253098 @default.
• W3085292324 cites W2139930584 @default.
• W3085292324 cites W2142873050 @default.
• W3085292324 cites W2144553406 @default.
• W3085292324 cites W2145457496 @default.
• W3085292324 cites W2148312526 @default.
• W3085292324 cites W2149525061 @default.
• W3085292324 cites W2152301430 @default.
• W3085292324 cites W2154626303 @default.
• W3085292324 cites W2154714625 @default.
• W3085292324 cites W2156999424 @default.
• W3085292324 cites W2157826046 @default.
• W3085292324 cites W2160586365 @default.
• W3085292324 cites W2163336852 @default.
• W3085292324 cites W2176616113 @default.
• W3085292324 cites W2180229411 @default.
• W3085292324 cites W2313905667 @default.
• W3085292324 cites W2586432482 @default.
• W3085292324 cites W2606439133 @default.
• W3085292324 cites W2620106353 @default.
• W3085292324 cites W2735451764 @default.
• W3085292324 cites W2791546842 @default.
• W3085292324 cites W2804822363 @default.
• W3085292324 cites W2807107167 @default.
• W3085292324 cites W2876540176 @default.
• W3085292324 cites W2890254886 @default.
• W3085292324 cites W2912290813 @default.
• W3085292324 cites W2951473001 @default.

• next