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- W3085312076 abstract "The assembly of centromeric regions has become one of the most intractable tasks in whole-genome sequencing due to the enrichment of highly repetitive DNA sequences in most eukaryotic centromeres. Here, we describe a method used to identify centromeric DNAs through chromatin immunoprecipitation and sequencing (ChIP-seq). By mapping ChIP-seq reads, centromeric regions can be indicated in genome assemblies. We demonstrated that the assembly quality of centromeres obtained using ChIP-seq mapping can reflect and indicate the quality of a whole-genome assembly. We discuss an expected 'high-quality' centromere assembly obtained via centromere ChIP-seq mapping." @default.
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- W3085312076 date "2021-03-01" @default.
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- W3085312076 title "Considerations regarding centromere assembly in plant whole-genome sequencing" @default.
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- W3085312076 doi "https://doi.org/10.1016/j.ymeth.2020.09.006" @default.
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