Matches in SemOpenAlex for { <https://semopenalex.org/work/W3085752747> ?p ?o ?g. }
- W3085752747 abstract "ABSTRACT We present a comprehensive, experimental and theoretical study of the impact of 5-hydroxymethylation of DNA cytosine. Using molecular dynamics, biophysical experiments and NMR spectroscopy, we found that Ten-Eleven translocation (TET) dioxygenases generate an epigenetic variant with structural and physical properties not too different to those of 5-methylcytosine. Experiments and simulations demonstrate that 5-methyl-cytosine (mC) and 5-hydroxymethyl-cytosine (hmC) generally lead to more rigid duplexes with poorer circularization efficiencies and lower ability to form nucleosomes. In particular, we can rule out the hypothesis that hydroxymethylation reverts to unmodified cytosine physical properties, as hmC is even more rigid than mC. Thus, we do not expect dramatic changes in the chromatin structure induced by differences in physical properties between d(mCpG) and d(hmCpG). On the contrary, our simulations suggest that methylated-DNA binding domains (MBD), associated with repression activities, are very sensitive to the substitution d(mCpG)→ d(hmCpG), while MBD3 which has a dual activation/repression activity is not sensitive to the d(mCpG) → d(hmCpG) change. Overall, while changes in gene activity due to cytosine methylation are the result of the combination of stiffness-related chromatin reorganization and MBD binding, those associated to 5-hydroxylation of methylcytosine could be explained by a change in the balance of repression/activation pathways related to differential MBD binding." @default.
- W3085752747 created "2020-09-21" @default.
- W3085752747 creator A5006399866 @default.
- W3085752747 creator A5038601330 @default.
- W3085752747 creator A5043833446 @default.
- W3085752747 creator A5050161114 @default.
- W3085752747 creator A5053570879 @default.
- W3085752747 creator A5054488801 @default.
- W3085752747 creator A5062566652 @default.
- W3085752747 creator A5070510752 @default.
- W3085752747 creator A5073481976 @default.
- W3085752747 creator A5090472903 @default.
- W3085752747 date "2020-09-17" @default.
- W3085752747 modified "2023-10-16" @default.
- W3085752747 title "The Impact of The Hydroxymethyl-Cytosine Epigenetic Signature on DNA Structure And Function" @default.
- W3085752747 cites W160347429 @default.
- W3085752747 cites W1603840783 @default.
- W3085752747 cites W1965932123 @default.
- W3085752747 cites W1967064280 @default.
- W3085752747 cites W1969776708 @default.
- W3085752747 cites W1971157495 @default.
- W3085752747 cites W1975510301 @default.
- W3085752747 cites W1976499671 @default.
- W3085752747 cites W1977520105 @default.
- W3085752747 cites W1981543052 @default.
- W3085752747 cites W1982255060 @default.
- W3085752747 cites W1989457196 @default.
- W3085752747 cites W1997453048 @default.
- W3085752747 cites W1998517881 @default.
- W3085752747 cites W2006208344 @default.
- W3085752747 cites W2010984732 @default.
- W3085752747 cites W2017141862 @default.
- W3085752747 cites W2017668896 @default.
- W3085752747 cites W2020016210 @default.
- W3085752747 cites W2027555611 @default.
- W3085752747 cites W2029667189 @default.
- W3085752747 cites W2029742174 @default.
- W3085752747 cites W2029746613 @default.
- W3085752747 cites W2032183039 @default.
- W3085752747 cites W2032882684 @default.
- W3085752747 cites W2035266068 @default.
- W3085752747 cites W2038449573 @default.
- W3085752747 cites W2038722690 @default.
- W3085752747 cites W2038756976 @default.
- W3085752747 cites W2039870229 @default.
- W3085752747 cites W2040875973 @default.
- W3085752747 cites W2043472013 @default.
- W3085752747 cites W2044987317 @default.
- W3085752747 cites W2046812959 @default.
- W3085752747 cites W2047246007 @default.
- W3085752747 cites W2056927568 @default.
- W3085752747 cites W2058093052 @default.
- W3085752747 cites W2064432113 @default.
- W3085752747 cites W2067174909 @default.
- W3085752747 cites W2072301590 @default.
- W3085752747 cites W2076255761 @default.
- W3085752747 cites W2080871860 @default.
- W3085752747 cites W2080945020 @default.
- W3085752747 cites W2081195534 @default.
- W3085752747 cites W2093450917 @default.
- W3085752747 cites W2095994064 @default.
- W3085752747 cites W2097342705 @default.
- W3085752747 cites W2108664872 @default.
- W3085752747 cites W2111538587 @default.
- W3085752747 cites W2114970231 @default.
- W3085752747 cites W2123032740 @default.
- W3085752747 cites W2129745108 @default.
- W3085752747 cites W2131684560 @default.
- W3085752747 cites W2132629607 @default.
- W3085752747 cites W2149119305 @default.
- W3085752747 cites W2149263899 @default.
- W3085752747 cites W2152451432 @default.
- W3085752747 cites W2152497434 @default.
- W3085752747 cites W2152636479 @default.
- W3085752747 cites W2156386262 @default.
- W3085752747 cites W2157458089 @default.
- W3085752747 cites W2161098391 @default.
- W3085752747 cites W2170516638 @default.
- W3085752747 cites W2170891278 @default.
- W3085752747 cites W2174058075 @default.
- W3085752747 cites W2202710059 @default.
- W3085752747 cites W2340786023 @default.
- W3085752747 cites W2903726264 @default.
- W3085752747 cites W2991285488 @default.
- W3085752747 doi "https://doi.org/10.1101/2020.09.17.285452" @default.
- W3085752747 hasPublicationYear "2020" @default.
- W3085752747 type Work @default.
- W3085752747 sameAs 3085752747 @default.
- W3085752747 citedByCount "0" @default.
- W3085752747 crossrefType "posted-content" @default.
- W3085752747 hasAuthorship W3085752747A5006399866 @default.
- W3085752747 hasAuthorship W3085752747A5038601330 @default.
- W3085752747 hasAuthorship W3085752747A5043833446 @default.
- W3085752747 hasAuthorship W3085752747A5050161114 @default.
- W3085752747 hasAuthorship W3085752747A5053570879 @default.
- W3085752747 hasAuthorship W3085752747A5054488801 @default.
- W3085752747 hasAuthorship W3085752747A5062566652 @default.
- W3085752747 hasAuthorship W3085752747A5070510752 @default.
- W3085752747 hasAuthorship W3085752747A5073481976 @default.
- W3085752747 hasAuthorship W3085752747A5090472903 @default.