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• W3085853125 abstract "Abstract Background Crude glycerol as a promising feedstock for microbial lipid production contains several impurities that make it toxic stress inducer at high amount. Under stress conditions, microorganisms can accumulate l -proline as a safeguard. Herein, l -proline was assessed as an anti-stress agent in crude glycerol media. Results Crude glycerol was converted to microbial lipids by the oleaginous yeast Rhodosporidium toruloides CGMCC 2.1389 in a two-staged culture mode. The media was supplied with exogenous l -proline to improve lipid production efficiency in high crude glycerol stress. An optimal amount of 0.5 g/L l -proline increased lipid titer and lipid yield by 34% and 28%, respectively. The lipid titer of 12.2 g/L and lipid content of 64.5% with a highest lipid yield of 0.26 g/g were achieved with l -proline addition, which were far higher than those of the control, i.e., lipid titer of 9.1 g/L, lipid content of 58% and lipid yield of 0.21 g/g. Similarly, l -proline also improved cell growth and glycerol consumption. Moreover, fatty acid compositional profiles of the lipid products was found suitable as a potential feedstock for biodiesel production. Conclusion Our study suggested that exogenous l -proline improved cell growth and lipid production on crude glycerol by R. toruloides . The fact that higher lipid yield as well as glycerol consumption indicated that l -proline might act as a potential anti-stress agent for the oleaginous yeast strain." @default.
• W3085853125 created "2020-09-21" @default.
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• W3085853125 date "2020-09-14" @default.
• W3085853125 modified "2023-09-25" @default.
• W3085853125 title "Exogenous l-proline improved Rhodosporidium toruloides lipid production on crude glycerol" @default.
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• W3085853125 doi "https://doi.org/10.1186/s13068-020-01798-6" @default.
• W3085853125 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7490893" @default.
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