Matches in SemOpenAlex for { <https://semopenalex.org/work/W3086095145> ?p ?o ?g. }
- W3086095145 abstract "Silkworm ovarian germ cells produce the Siwi-piRNA-induced silencing complex (piRISC) through two consecutive mechanisms, the primary pathway and the secondary ping-pong cycle. Primary Siwi-piRISC production occurs on the outer mitochondrial membrane in an Ago3-independent manner, where Tudor domain-containing Papi binds unloaded Siwi via its symmetrical dimethylarginines (sDMAs). Here, we now show that secondary Siwi-piRISC production occurs at the Ago3-positive nuage Ago3 bodies, in an Ago3-dependent manner, where Vreteno (Vret), another Tudor protein, interconnects unloaded Siwi and Ago3-piRISC through their sDMAs. Upon Siwi depletion, Ago3 is phosphorylated and insolubilized in its piRISC form with cleaved RNAs and Vret, suggesting that the complex is stalled in the intermediate state. The Ago3 bodies are also enlarged. The aberrant morphology is restored upon Siwi re-expression without Ago3-piRISC supply. Thus, Siwi depletion aggregates the Ago3 bodies to protect the piRNA intermediates from degradation until the normal cellular environment returns to re-initiate the ping-pong cycle. Overall, these findings reveal a unique regulatory mechanism controlling piRNA biogenesis." @default.
- W3086095145 created "2020-09-21" @default.
- W3086095145 creator A5009078832 @default.
- W3086095145 creator A5014010927 @default.
- W3086095145 creator A5016806376 @default.
- W3086095145 creator A5037597031 @default.
- W3086095145 creator A5044697505 @default.
- W3086095145 creator A5066704546 @default.
- W3086095145 creator A5071116943 @default.
- W3086095145 creator A5071569394 @default.
- W3086095145 date "2020-09-11" @default.
- W3086095145 modified "2023-10-12" @default.
- W3086095145 title "Siwi levels reversibly regulate secondary pi <scp>RISC</scp> biogenesis by affecting Ago3 body morphology in <i>Bombyx mori</i>" @default.
- W3086095145 cites W1528632786 @default.
- W3086095145 cites W1559389849 @default.
- W3086095145 cites W1596971406 @default.
- W3086095145 cites W1608004664 @default.
- W3086095145 cites W1659482056 @default.
- W3086095145 cites W1709210465 @default.
- W3086095145 cites W1971549481 @default.
- W3086095145 cites W1973596898 @default.
- W3086095145 cites W1976342457 @default.
- W3086095145 cites W1977733607 @default.
- W3086095145 cites W1988823631 @default.
- W3086095145 cites W1990654003 @default.
- W3086095145 cites W1992818586 @default.
- W3086095145 cites W2009644747 @default.
- W3086095145 cites W2015231728 @default.
- W3086095145 cites W2028118471 @default.
- W3086095145 cites W2028995073 @default.
- W3086095145 cites W2035303830 @default.
- W3086095145 cites W2036963953 @default.
- W3086095145 cites W2044545834 @default.
- W3086095145 cites W2051275283 @default.
- W3086095145 cites W2054343514 @default.
- W3086095145 cites W205686078 @default.
- W3086095145 cites W2056938017 @default.
- W3086095145 cites W2058939605 @default.
- W3086095145 cites W2072795057 @default.
- W3086095145 cites W2077050148 @default.
- W3086095145 cites W2096196036 @default.
- W3086095145 cites W2098715150 @default.
- W3086095145 cites W2101708445 @default.
- W3086095145 cites W2104582743 @default.
- W3086095145 cites W2105097906 @default.
- W3086095145 cites W2113377893 @default.
- W3086095145 cites W2115829042 @default.
- W3086095145 cites W2131489670 @default.
- W3086095145 cites W2132102978 @default.
- W3086095145 cites W2142754990 @default.
- W3086095145 cites W2142965408 @default.
- W3086095145 cites W2151741487 @default.
- W3086095145 cites W2157334713 @default.
- W3086095145 cites W2157378797 @default.
- W3086095145 cites W2157663132 @default.
- W3086095145 cites W2161115104 @default.
- W3086095145 cites W2165495041 @default.
- W3086095145 cites W2166871032 @default.
- W3086095145 cites W2168639701 @default.
- W3086095145 cites W2526067614 @default.
- W3086095145 cites W2590568317 @default.
- W3086095145 cites W2768687709 @default.
- W3086095145 cites W2776565218 @default.
- W3086095145 cites W2788003276 @default.
- W3086095145 cites W2901318957 @default.
- W3086095145 cites W2903232440 @default.
- W3086095145 cites W2942638147 @default.
- W3086095145 cites W2983030753 @default.
- W3086095145 cites W2996407735 @default.
- W3086095145 cites W2999685656 @default.
- W3086095145 cites W3006327256 @default.
- W3086095145 doi "https://doi.org/10.15252/embj.2020105130" @default.
- W3086095145 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7560202" @default.
- W3086095145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32914505" @default.
- W3086095145 hasPublicationYear "2020" @default.
- W3086095145 type Work @default.
- W3086095145 sameAs 3086095145 @default.
- W3086095145 citedByCount "8" @default.
- W3086095145 countsByYear W30860951452021 @default.
- W3086095145 countsByYear W30860951452022 @default.
- W3086095145 countsByYear W30860951452023 @default.
- W3086095145 crossrefType "journal-article" @default.
- W3086095145 hasAuthorship W3086095145A5009078832 @default.
- W3086095145 hasAuthorship W3086095145A5014010927 @default.
- W3086095145 hasAuthorship W3086095145A5016806376 @default.
- W3086095145 hasAuthorship W3086095145A5037597031 @default.
- W3086095145 hasAuthorship W3086095145A5044697505 @default.
- W3086095145 hasAuthorship W3086095145A5066704546 @default.
- W3086095145 hasAuthorship W3086095145A5071116943 @default.
- W3086095145 hasAuthorship W3086095145A5071569394 @default.
- W3086095145 hasBestOaLocation W30860951451 @default.
- W3086095145 hasConcept C104317684 @default.
- W3086095145 hasConcept C105702510 @default.
- W3086095145 hasConcept C131934819 @default.
- W3086095145 hasConcept C153911025 @default.
- W3086095145 hasConcept C2778487026 @default.
- W3086095145 hasConcept C499950583 @default.
- W3086095145 hasConcept C54355233 @default.
- W3086095145 hasConcept C86803240 @default.
- W3086095145 hasConcept C90856448 @default.