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• W3086487376 abstract "Felids play an important role in the transmission of Toxoplasma gondii to humans and other animals since they can excrete millions of oocysts into the environment as definitive hosts. In the present study, seroprevalence and risk factors of feline Toxoplasma infection were investigated, and molecular identification was conducted for T. gondii oocysts isolated from faecal samples of seropositive cats. A total of 276 cat serum samples collected from the Yangon, Myanmar were tested for T. gondii antibodies by ELISA. The overall seroprevalence of T. gondii infection was 41.30% (114 seropositive cats). Age between 1 and 6 years (OR = 3.284; 95% CI = 1.462–7.375), age > 6 years (OR = 4.560; 95% CI = 1.588–13.100) and sex (OR = 1.725; 95% CI = 1.026–2.899) were found to be significant (P < 0.05) factors associated with T. gondii infection. DNA samples extracted from a single oocyst of seropositive cats were employed in three PCR assays amplifying parasite TOX-element and mitochondrial COI, and SAG2 locus. The obtained sequences of TOX-elements (n = 6) and COI (n = 5) were identical to those of T. gondii previously deposited in Genbank. SAG2 PCR yielded three different sequences, all of which were clustered with Type I T. gondii isolates in a phylogenetic tree. This study reported the seroprevalence and risk factors for T. gondii infection in cats and provided the molecular information on the parasite in Myanmar." @default.
• W3086487376 created "2020-09-21" @default.
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• W3086487376 date "2020-09-01" @default.
• W3086487376 modified "2023-10-16" @default.
• W3086487376 title "Seroprevalence of Toxoplasma gondii in household cats in Myanmar and molecular identification of parasites using feline faecal oocysts" @default.
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• W3086487376 doi "https://doi.org/10.1016/j.fawpar.2020.e00094" @default.
• W3086487376 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7502821" @default.
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