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- W3086918963 endingPage "147" @default.
- W3086918963 startingPage "91" @default.
- W3086918963 abstract "The biochemical differences between tissues fixed with cross-linking fixatives (formalin, paraformaldehyde, glutaraldehyde) and those fixed in denaturing fixatives (Carnoy’s, acetone, alcohols, and acetic acid) or unfixed samples are profound. An understanding of this point allows you to better understand the marked differences in signal strength, subcellular localization, and coexpression capability when comparing these two broad categories of tissues. This chapter takes you on a journey through each step used during in situ hybridization and focuses on the biochemical explanations regarding why variables such as the type of probe, the type of fixative, and the wash are so important for optimal results. Further, this chapter introduces the phenomenon that different formalin-fixed, paraffin-embedded tissues, even if they share the same exact histologic diagnosis, may present very different three-dimensional biochemical profiles, which can have a strong impact on the optimal pretreatment condition for in situ hybridization." @default.
- W3086918963 created "2020-09-21" @default.
- W3086918963 creator A5081369976 @default.
- W3086918963 date "2021-01-01" @default.
- W3086918963 modified "2023-10-09" @default.
- W3086918963 title "The basics of in situ hybridization" @default.
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