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• W3087579302 endingPage "128803" @default.
• W3087579302 startingPage "128803" @default.
• W3087579302 abstract "In recent years, need for rapid, reliable, and low-cost protein biomarker screening has tremendously boosted research into the development of electrochemical immunosensors. In this line, here we report on rapid fabrication and proof-of-concept demonstration of a hyper-branched gold nanostructure ([email protected]) based electrochemical immunosensor for detection of protein biomarker at pg/mL level. We have optimized a simple direct electrochemical process to rapidly generate uniformly distributed high surface area [email protected] within 30 min. These 3D [email protected] show ∼ 8-times enhancement in conductivity and also a rapid surface functionalization via self-assembled monolayers (SAM) of mercaptoundecanoic acid within 45 min. Protein estimation data showed around 94 % immobilization efficiency on the [email protected] surface in comparison to 28.4 % on plain gold surface. A fast SAM formation and quick antibody immobilization steered the assay timing within 4 h. As a proof-of-concept, we used an antibody-antigen interaction for the detection of epithelial growth factor receptor (EGFR) protein. Voltammetric data for the target EGFR receptor resulted a linear working range of 10 pg/mL to 100 ng/mL with a low limit-of-detection of 6.9 pg/mL. High selectivity in detecting target proteins from a pool of non-target proteins using breast cancer cell lines was also demonstrated along with good stability and reusability." @default.
• W3087579302 created "2020-09-25" @default.
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• W3087579302 date "2021-01-01" @default.
• W3087579302 modified "2023-10-09" @default.
• W3087579302 title "Rapid fabrication of homogeneously distributed hyper-branched gold nanostructured electrode based electrochemical immunosensor for detection of protein biomarkers" @default.
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• W3087579302 doi "https://doi.org/10.1016/j.snb.2020.128803" @default.
• W3087579302 hasPublicationYear "2021" @default.
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