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- W3087617615 abstract "Abstract Plants possess unique primary cell walls made of complex polysaccharides that play critical roles in determining intrinsic cell and organ size. How genes responsible for synthesizing and modifying the polysaccharides are regulated by microRNAs (miRNAs) to control plant size remains largely unexplored. Here we identified 23 putative cell wall related miRNAs, termed CW-miRNAs, in Arabidopsis thaliana and characterized miR775 as an example. We showed that miR775 post-transcriptionally silences GALT9 , which encodes an endomembrane-located galactosyltransferase belonging to the glycosyltransferase 31 family. Over-expression of miR775 and deletion of GALT9 significantly enlarged leaf-related organs, primarily owing to increases in cell size. Monosaccharide quantification, confocal Raman imaging, and immunolabelling combined with atomic force microscopy (AFM) revealed that the MIR775A - GALT9 circuit modulates pectin level and elastic modulus of the cell wall. We further showed that MIR775A is directly repressed by the transcription factor ELONGATED HYPOCOTYL 5 (HY5). Genetic analysis confirmed that HY5 is a negative regulator of leaf size and acts through the HY5-MIR775A-GALT9 repression cascade to control pectin level. These results demonstrate that miR775-regulated cell wall remodeling is an integral determinant for intrinsic leaf size in A. thaliana and highlight the need to study other CW-miRNAs for more insights into cell wall biology." @default.
- W3087617615 created "2020-09-25" @default.
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- W3087617615 date "2020-09-18" @default.
- W3087617615 modified "2023-09-26" @default.
- W3087617615 title "MicroRNA775 Promotes Intrinsic Leaf Size and Reduces Cell Wall Pectin Level via a Target Galactosyltransferase in<i>Arabidopsis</i>" @default.
- W3087617615 cites W1492034352 @default.
- W3087617615 cites W1501088252 @default.
- W3087617615 cites W1518650651 @default.
- W3087617615 cites W1610109254 @default.
- W3087617615 cites W1850376420 @default.
- W3087617615 cites W1978915732 @default.
- W3087617615 cites W1981629037 @default.
- W3087617615 cites W1988904746 @default.
- W3087617615 cites W1992873592 @default.
- W3087617615 cites W1993071481 @default.
- W3087617615 cites W1994688090 @default.
- W3087617615 cites W1995973955 @default.
- W3087617615 cites W2001396309 @default.
- W3087617615 cites W2018403717 @default.
- W3087617615 cites W2021142610 @default.
- W3087617615 cites W2024530550 @default.
- W3087617615 cites W2025087195 @default.
- W3087617615 cites W2030489111 @default.
- W3087617615 cites W2033667297 @default.
- W3087617615 cites W2042325491 @default.
- W3087617615 cites W2054708511 @default.
- W3087617615 cites W2056371419 @default.
- W3087617615 cites W2064379205 @default.
- W3087617615 cites W2064485022 @default.
- W3087617615 cites W2064672115 @default.
- W3087617615 cites W2065363143 @default.
- W3087617615 cites W2067364373 @default.
- W3087617615 cites W2067920743 @default.
- W3087617615 cites W2080157706 @default.
- W3087617615 cites W2080365565 @default.
- W3087617615 cites W2081261052 @default.
- W3087617615 cites W2082452795 @default.
- W3087617615 cites W2098195313 @default.
- W3087617615 cites W2099660828 @default.
- W3087617615 cites W2100885056 @default.
- W3087617615 cites W2104322803 @default.
- W3087617615 cites W2105686854 @default.
- W3087617615 cites W2106441153 @default.
- W3087617615 cites W2106728647 @default.
- W3087617615 cites W2108368616 @default.
- W3087617615 cites W2108979462 @default.
- W3087617615 cites W2109980254 @default.
- W3087617615 cites W2110636006 @default.
- W3087617615 cites W2118771503 @default.
- W3087617615 cites W2120080928 @default.
- W3087617615 cites W2124514540 @default.
- W3087617615 cites W2126685043 @default.
- W3087617615 cites W2128525869 @default.
- W3087617615 cites W2130242517 @default.
- W3087617615 cites W2130892638 @default.
- W3087617615 cites W2132690327 @default.
- W3087617615 cites W2133613866 @default.
- W3087617615 cites W2134914093 @default.
- W3087617615 cites W2135015580 @default.
- W3087617615 cites W2135959854 @default.
- W3087617615 cites W2142022187 @default.
- W3087617615 cites W2144110489 @default.
- W3087617615 cites W2144177541 @default.
- W3087617615 cites W2145114408 @default.
- W3087617615 cites W2145839489 @default.
- W3087617615 cites W2146860118 @default.
- W3087617615 cites W2152324471 @default.
- W3087617615 cites W2154241726 @default.
- W3087617615 cites W2157953344 @default.
- W3087617615 cites W2158794108 @default.
- W3087617615 cites W2166955792 @default.
- W3087617615 cites W2167259011 @default.
- W3087617615 cites W2167549712 @default.
- W3087617615 cites W2168803371 @default.
- W3087617615 cites W2170551349 @default.
- W3087617615 cites W2170561036 @default.
- W3087617615 cites W2256186434 @default.
- W3087617615 cites W2301701784 @default.
- W3087617615 cites W2544557857 @default.
- W3087617615 cites W2554351419 @default.
- W3087617615 cites W2555776926 @default.
- W3087617615 cites W2729054356 @default.
- W3087617615 cites W2750779169 @default.
- W3087617615 cites W2762930692 @default.
- W3087617615 cites W2775315818 @default.
- W3087617615 cites W2783753653 @default.