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- W3088056688 abstract "We used ribosome profiling to characterize the biological role of ribosome recycling factor (RRF) in Escherichia coli . As expected, RRF depletion leads to enrichment of post-termination 70S complexes in 3′-UTRs. We also observe that elongating ribosomes are unable to complete translation because they are blocked by non-recycled ribosomes at stop codons. Previous studies have suggested a role for recycling in translational coupling within operons; if a ribosome remains bound to an mRNA after termination, it may re-initiate downstream. We found, however, that RRF depletion did not significantly affect coupling efficiency in reporter assays or in ribosome density genome-wide. These findings argue that re-initiation is not a major mechanism of translational coupling in E. coli . Finally, RRF depletion has dramatic effects on the activity of ribosome rescue factors tmRNA and ArfA. Our results provide a global view of the effects of the loss of ribosome recycling on protein synthesis in E. coli." @default.
- W3088056688 created "2020-10-01" @default.
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- W3088056688 date "2020-09-23" @default.
- W3088056688 modified "2023-09-26" @default.
- W3088056688 title "Ribosome recycling is not critical for translational coupling in Escherichia coli" @default.
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- W3088056688 doi "https://doi.org/10.7554/elife.59974" @default.
- W3088056688 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7538156" @default.
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