FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3088183035> ?p ?o ?g. }

• W3088183035 endingPage "112647" @default.
• W3088183035 startingPage "112647" @default.
• W3088183035 abstract "Protease expression is closely linked to various pathological phenomena, and their accurate quantification is essential to clinical diagnosis and cancer therapy. Herein, we demonstrate for the first time the construction of a sensitive protease sensor by integrating protease-sensitive cleavage with nicking enzyme-assisted signal amplification (NESA) for single-molecule detection of multiple matrix metalloproteinases (MMPs). This protease sensor involves two DNA-peptide conjugates which contain both specific protease cleavage sites and trigger DNAs and two report DNAs which are modified with a fluorophore (Cy3 or Cy5) and a quencher (BHQ2). In the presence of specific MMPs, MMPs-mediated cleavage reactions lead to the release of specific trigger DNAs from the corresponding DNA-peptide conjugates. After the magnetic separation, the resultant trigger DNAs may hybridize with the corresponding report DNAs to initiate the cyclic NESA reaction, releasing large amounts of Cy3/Cy5 fluorescent molecules which can be simply quantified by using total internal reflection fluorescence-based single-molecule detection. Taking advantage of the high specificity of proteolytic cleavage, the high amplification efficiency of cyclic NESA, and the high sensitivity of single-molecule detection, this protease sensor can simultaneously detect multiple MMPs with a detection limit of 3.33 pM for MMP-2 and 1.71 pM for MMP-7, superior to the target peptide-based methods. Moreover, this protease sensor can be applied for the measurement of MMP-2 and MMP-7 in cancer cells and the screening of protease inhibitors, holding great promise in clinic diagnosis and drug discovery." @default.
• W3088183035 created "2020-10-01" @default.
• W3088183035 creator A5001568746 @default.
• W3088183035 creator A5001969147 @default.
• W3088183035 creator A5024463173 @default.
• W3088183035 creator A5079043288 @default.
• W3088183035 creator A5079701813 @default.
• W3088183035 date "2020-12-01" @default.
• W3088183035 modified "2023-10-18" @default.
• W3088183035 title "Construction of a sensitive protease sensor with DNA-peptide conjugates for single-molecule detection of multiple matrix metalloproteinases" @default.
• W3088183035 cites W1533605450 @default.
• W3088183035 cites W1555862556 @default.
• W3088183035 cites W1572736799 @default.
• W3088183035 cites W1575075003 @default.
• W3088183035 cites W1966081795 @default.
• W3088183035 cites W1989389380 @default.
• W3088183035 cites W1990952589 @default.
• W3088183035 cites W1997104566 @default.
• W3088183035 cites W2003063710 @default.
• W3088183035 cites W2003500892 @default.
• W3088183035 cites W2004034027 @default.
• W3088183035 cites W2004260843 @default.
• W3088183035 cites W2007066284 @default.
• W3088183035 cites W2008690139 @default.
• W3088183035 cites W2009172139 @default.
• W3088183035 cites W2050834003 @default.
• W3088183035 cites W2053559643 @default.
• W3088183035 cites W2055758035 @default.
• W3088183035 cites W2056975585 @default.
• W3088183035 cites W2067262804 @default.
• W3088183035 cites W2075114388 @default.
• W3088183035 cites W2078386086 @default.
• W3088183035 cites W2081667191 @default.
• W3088183035 cites W2082848986 @default.
• W3088183035 cites W2083535354 @default.
• W3088183035 cites W2088422531 @default.
• W3088183035 cites W2093512107 @default.
• W3088183035 cites W2098168395 @default.
• W3088183035 cites W2101353026 @default.
• W3088183035 cites W2115282334 @default.
• W3088183035 cites W2119150902 @default.
• W3088183035 cites W2128049766 @default.
• W3088183035 cites W2149560261 @default.
• W3088183035 cites W2154401382 @default.
• W3088183035 cites W2164564532 @default.
• W3088183035 cites W2277082392 @default.
• W3088183035 cites W2326184101 @default.
• W3088183035 cites W2519581666 @default.
• W3088183035 cites W2614658780 @default.
• W3088183035 cites W2618426087 @default.
• W3088183035 cites W2626782461 @default.
• W3088183035 cites W2767681889 @default.
• W3088183035 cites W2982404291 @default.
• W3088183035 cites W2984614812 @default.
• W3088183035 cites W3011193704 @default.
• W3088183035 cites W3013464670 @default.
• W3088183035 cites W4230860477 @default.
• W3088183035 cites W4250908540 @default.
• W3088183035 doi "https://doi.org/10.1016/j.bios.2020.112647" @default.
• W3088183035 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32992161" @default.
• W3088183035 hasPublicationYear "2020" @default.
• W3088183035 type Work @default.
• W3088183035 sameAs 3088183035 @default.
• W3088183035 citedByCount "15" @default.
• W3088183035 countsByYear W30881830352020 @default.
• W3088183035 countsByYear W30881830352021 @default.
• W3088183035 countsByYear W30881830352022 @default.
• W3088183035 countsByYear W30881830352023 @default.
• W3088183035 crossrefType "journal-article" @default.
• W3088183035 hasAuthorship W3088183035A5001568746 @default.
• W3088183035 hasAuthorship W3088183035A5001969147 @default.
• W3088183035 hasAuthorship W3088183035A5024463173 @default.
• W3088183035 hasAuthorship W3088183035A5079043288 @default.
• W3088183035 hasAuthorship W3088183035A5079701813 @default.
• W3088183035 hasConcept C109523444 @default.
• W3088183035 hasConcept C121332964 @default.
• W3088183035 hasConcept C12554922 @default.
• W3088183035 hasConcept C151730666 @default.
• W3088183035 hasConcept C153911025 @default.
• W3088183035 hasConcept C175156509 @default.
• W3088183035 hasConcept C181199279 @default.
• W3088183035 hasConcept C182220744 @default.
• W3088183035 hasConcept C185592680 @default.
• W3088183035 hasConcept C19014433 @default.
• W3088183035 hasConcept C2776714187 @default.
• W3088183035 hasConcept C2778617687 @default.
• W3088183035 hasConcept C2779281246 @default.
• W3088183035 hasConcept C2781307694 @default.
• W3088183035 hasConcept C3019223276 @default.
• W3088183035 hasConcept C43369102 @default.
• W3088183035 hasConcept C552990157 @default.
• W3088183035 hasConcept C55493867 @default.
• W3088183035 hasConcept C62520636 @default.
• W3088183035 hasConcept C86803240 @default.
• W3088183035 hasConcept C91881484 @default.
• W3088183035 hasConceptScore W3088183035C109523444 @default.
• W3088183035 hasConceptScore W3088183035C121332964 @default.
• W3088183035 hasConceptScore W3088183035C12554922 @default.

• next