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• W3088201933 endingPage "106144" @default.
• W3088201933 startingPage "106144" @default.
• W3088201933 abstract "• PET massive waste threatens marine animals and humans life. • Active and thermostable PET biocatalysts are needed for environment-friendly PET waste recycling. • Bioengineering strategies have a significant effect on biocatalysts’ production, activity, and stability. • Immobilization significantly reduces the production cost of biocatalysts via their reusability. • Practical and commercial applications of PET biocatalysts are in high demand. The massive waste of poly(ethylene terephthalate) (PET) that ends up in the landfills and oceans and needs hundreds of years for degradation has attracted global concern. The poor stability and productivity of the available PET biocatalysts hinder their industrial applications. Active PET biocatalysts can provide a promising avenue for PET bioconversion and recycling. Therefore, there is an urgent need to develop new strategies that could enhance the stability, catalytic activity, solubility, productivity, and re-usability of these PET biocatalysts under harsh conditions such as high temperatures, pH, and salinity. This has raised great attention in using bioengineering strategies to improve PET biocatalysts’ robustness and catalytic behavior. Herein, historical and forecasting data of plastic production and disposal were critically reviewed. Challenges facing the PET degradation process and available strategies that could be used to solve them were critically highlighted and summarized. In this review, we also discussed the recent progress in enzyme bioengineering approaches used for discovering new PET biocatalysts, elucidating the degradation mechanism, and improving the catalytic performance, solubility, and productivity, critically assess their strength and weakness and highlighting the gaps of the available data. Discovery of more potential PET hydrolases and studying their molecular mechanism extensively via solving their crystal structure will widen this research area to move forward the industrial application. A deeper knowledge of PET molecular and degradation mechanisms will give great insight into the future identification of related enzymes. The reported bioengineering strategies during this review could be used to reduce PET crystallinity and to increase the operational temperature of PET hydrolyzing enzymes." @default.
• W3088201933 created "2020-10-01" @default.
• W3088201933 creator A5012422842 @default.
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• W3088201933 creator A5046427631 @default.
• W3088201933 creator A5052752523 @default.
• W3088201933 creator A5088372944 @default.
• W3088201933 date "2020-12-01" @default.
• W3088201933 modified "2023-09-29" @default.
• W3088201933 title "Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling" @default.
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