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- W308820269 abstract "A potyvirus-like agent (isolate CY) was isolated from sweet potato with leaf symptoms of chlorotic spots and vein mottling in Chia-Yi area, Taiwan. A 1.9-kb DNA product was amplified by reverse transcription polymerase chain reaction (RT-PCR) from infected tissues of C. quinoa using the oligo (dT) and degenerate primers for potyviruses. cloned, and then sequenced. The cDNA fragment reflected 1880 nucleotides (nts) corresponding to the 3'-terminal region of a potyvirus and the sequence comparison indicated that the isolate CY belongs to Stint potato latent virus (SPLV). The deduced amino acid sequence was determined as 561 residues that contains a part of the Y terminal region of NIb gene (268 residues) and the complete .sequence of coat protein (CP) gene (293 residue). The 3-terminal region of the cDNA was determined containing a non-coding region (NCR) of 197 nts. The DAG triplet for aphid transmissibility was found at the 7-9 residues from the N terminus of CP gene. Multiple sequence alignment of the known sequences of SPLV indicated that the CP gene and the NCR of CY isolate share 96.5 and l00% nucleotide identities, respectively, with those of SPLV-TW. The phylogenetic analysis indicated that the CY isolate is closely related to Taiwan and China isolates, but distantly related to a Japan isolate. When a pair of specific primers designed from the CP gene of SPLV-CY was used for detection by RT-PCR, a 675 bp specific DNA fragment was amplified rout SPLV-CY infected plants." @default.
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- W308820269 date "2007-09-01" @default.
- W308820269 modified "2023-09-23" @default.
- W308820269 title "Nucleotide sequence analysis and detection application of the coat protein gene of Sweet potato latent virus isolated from central Taiwan." @default.
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